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Further Characterisation of the Molecular Signature of Quiescent and Activated Mouse Muscle Satellite Cells

By Viola F. Gnocchi, Robert B. White, Yusuke Ono, Juliet A. Ellis and Peter S. Zammit


Satellite cells are the resident stem cells of adult skeletal muscle. To date though, there is a paucity of native markers that can be used to easily identify quiescent satellite cells, with Pax7 probably being the best that is currently available. Here we have further characterized a number of recently described satellite cell markers, and also describe novel ones. Caveolin-1, integrin α7 and the calcitonin receptor proved reliable markers for quiescent satellite cells, being expressed by all satellite cells identified with Pax7. These three markers remained expressed as satellite cells were activated and underwent proliferation. The nuclear envelope proteins lamin A/C and emerin, mutations in which underlie Emery-Dreifuss muscular dystrophy, were also expressed in both quiescent and proliferating satellite cells. Conversely, Jagged-1, a Notch ligand, was not expressed in quiescent satellite cells but was induced upon activation. These findings further contribute to defining the molecular signature of muscle satellite cells

Topics: Research Article
Publisher: Public Library of Science
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Provided by: PubMed Central

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  11. (2006). Emery-Dreifuss muscular dystrophy at the nuclear envelope: 10 years on.
  12. (1990). Expression of myosin isoforms during notexin-induced regeneration of rat soleus muscles.
  13. (2004). Frequency of M-cadherin-stained satellite cells declines in human muscles during aging.
  14. (1992). Gamma irradiation prevents compensatory hypertrophy of overloaded mouse extensor digitorum longus muscle.
  15. (1994). Identification of a novel X-linked gene responsible for Emery-Dreifuss muscular dystrophy.
  16. (1995). Jagged: a mammalian ligand that activates Notch1.
  17. (1995). Kaminska A
  18. (2002). Kinetics of myoblast proliferation show that resident satellite cells are competent to fully regenerate skeletal muscle fibers.
  19. (2000). majority of quiescent adult skeletal muscle satellite cells.
  20. (2007). Molecular signature of quiescent satellite cells in adult skeletal muscle.
  21. (2004). Muscle satellite cells adopt divergent fates: a mechanism for self-renewal?
  22. (1998). Mutations in the caveolin-3 gene cause autosomal dominant limb-girdle muscular dystrophy.
  23. (1999). Mutations in the gene encoding lamin A/C cause autosomal dominant Emery-Dreifuss muscular dystrophy.
  24. (1998). Mutations in the integrin alpha7 gene cause congenital myopathy.
  25. (2007). Nestin-GFP reporter expression defines the quiescent state of skeletal muscle satellite cells.
  26. (2008). Nuclear changes in skeletal muscle extend to satellite cells in autosomal dominant EmeryDreifuss muscular dystrophy/limb-girdle muscular dystrophy 1B. Neuromuscul Disord.
  27. (2000). Pax7 is required for the specification of myogenic satellite cells.
  28. (1997). Persistent expression of MNF identifies myogenic stem cells in postnatal muscles.
  29. (2004). Purification and cell-surface marker characterization of quiescent satellite cells from murine skeletal muscle by a novel monoclonal antibody.
  30. (1992). Regenerating and denervated human muscle fibers and satellite cells express neural cell adhesion molecule recognized by monoclonal antibodies to natural killer cells.
  31. (2004). Satellite and stem cells in muscle regeneration.
  32. (1961). Satellite cell of skeletal muscle fibers.
  33. (2008). Self-renewal and expansion of single transplanted muscle stem cells.
  34. (2004). Sox15 is required for skeletal muscle regeneration.
  35. (2003). Sox8 is a specific marker for muscle satellite cells and inhibits myogenesis.
  36. (2006). Sphingomyelin levels in the plasma membrane correlate with the activation state of muscle satellite cells.
  37. (2005). Stem cell function, self-renewal, and behavioral heterogeneity of cells from the adult muscle satellite cell niche.
  38. (2001). Syndecan-3 and syndecan-4 specifically mark skeletal muscle satellite cells and are implicated in satellite cell maintenance and muscle regeneration.
  39. (1994). Temporal expression of regulatory and structural muscle proteins during myogenesis of satellite cells on isolated adult rat fibers.
  40. (2006). The differentiation and morphogenesis of craniofacial muscles.
  41. (1986). The nuclear lamina is a meshwork of intermediate-type filaments.
  42. (2002). The regulation of Notch signaling controls satellite cell activation and cell fate determination in postnatal myogenesis.
  43. (2001). The skeletal muscle satellite cell: stem cell or son of stem cell?
  44. (1994). Wernig A

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