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Cis-acting elements in the promoter region of the human aldolase C gene

By Pasqualina Buono, Lisa de Conciliis, Eleonora Olivetta, Paola Izzo and Francesco Salvatore


AbstractWe investigated the cis-acting sequences involved in the expression of the human aldolase C gene by transient transfections into human neuroblastoma cells (SKNBE). We demonstrate that 420 bp of the 5'-flanking DNA direct at high efficiency the transcription of the CAT reporter gene. A deletion between − 420 bp and − 164 bp causes a 60% decrease of CAT activity. Gel shift and DNase I footprinting analyses revealed four protected elements: A, B, C and D. Competition analyses indicate that Spl or factors sharing a similar sequence specificity bind to elements A and B, but not to elements C and D. Sequence analysis shows a half palindromic ERE motif (GGTCA), in elements B and D. Region D binds a transactivating factor which appears also essential to stabilize the initiation complex

Publisher: Published by Elsevier B.V.
Year: 1993
DOI identifier: 10.1016/0014-5793(93)80936-O
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