Skip to main content
Article thumbnail
Location of Repository

Borrelia burgdorferi EbfC defines a newly-identified, widespread family of bacterial DNA-binding proteins

By Sean P. Riley, Tomasz Bykowski, Anne E. Cooley, Logan H. Burns, Kelly Babb, Catherine A. Brissette, Amy Bowman, Matthew Rotondi, M. Clarke Miller, Edward DeMoll, Kap Lim, Michael G. Fried and Brian Stevenson


The Lyme disease spirochete, Borrelia burgdorferi, encodes a novel type of DNA-binding protein named EbfC. Orthologs of EbfC are encoded by a wide range of bacterial species, so characterization of the borrelial protein has implications that span the eubacterial kingdom. The present work defines the DNA sequence required for high-affinity binding by EbfC to be the 4 bp broken palindrome GTnAC, where ‘n’ can be any nucleotide. Two high-affinity EbfC-binding sites are located immediately 5′ of B. burgdorferi erp transcriptional promoters, and binding of EbfC was found to alter the conformation of erp promoter DNA. Consensus EbfC-binding sites are abundantly distributed throughout the B. burgdorferi genome, occurring approximately once every 1 kb. These and other features of EbfC suggest that this small protein and its orthologs may represent a distinctive type of bacterial nucleoid-associated protein. EbfC was shown to bind DNA as a homodimer, and site-directed mutagenesis studies indicated that EbfC and its orthologs appear to bind DNA via a novel α-helical ‘tweezer’-like structure

Topics: Molecular Biology
Publisher: Oxford University Press
OAI identifier:
Provided by: PubMed Central
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://www.pubmedcentral.nih.g... (external link)
  • Suggested articles


    1. (2000). A bacterial genome in flux: the twelve linear and nine circular extrachromosomal DNAs of an infectious isolate of the Lyme disease spirochete Borrelia burgdorferi.
    2. (1996). A family of genes located on four separate 32-kilobase circular plasmids in Borrelia burgdorferi B31.
    3. (1988). A fast algorithm for rendering space-filling molecule pictures.
    4. (2000). A second allele of eppA in Borrelia burgdorferi strain B31 is located on the previously undetected circular plasmid cp9-2.
    5. (2007). Analysis of protein-DNA equilibria by native gel electrophoresis.
    6. (2003). Analysis of the OspE determinants involved in binding of factor H and OspE-targeting antibodies elicited during Borrelia burgdorferi infection.
    7. (1995). Association between different clinical manifestations of Lyme disease and different species of Borrelia burgdorferi sensu lato.
    8. (2006). Borrelia burgdorferi EbfC, a novel, chromosomally-encoded protein, binds specific DNA sequences adjacent to erp loci on the spirochete’s resident cp32 prophages.
    9. (2005). CDD: a conserved domain database for protein classification.
    10. (2001). Compaction of single DNA molecules induced by binding of integration host factor (IHF).
    11. (2002). Complement inhibitor factor H binding to Lyme disease spirochetes is mediated by inducible expression of multiple plasmid-encoded outer surface protein E paralogs.
    12. (2003). Crystal structure of YbaB from Haemophilus influenzae (HI0442), a protein of unknown function coexpressed with the recombinational DNA repair protein RecR.
    13. (1998). Crystallography & NMR system: a new software suite for macromolecular structure determination. Acta Crystallogr.
    14. (1993). Different genospecies of Borrelia burgdorferi are associated with distinct clinical manifestations of Lyme borreliosis.
    15. (2002). Differential binding of host complement inhibitor factor H by Borrelia burgdorferi Erp surface proteins: a possible mechanism underlying the expansive host range of Lyme disease spirochetes.
    16. (1996). DNA binding mechanism of O6-alkylguanine-DNA alkyltransferase: stoichiometry and effects of DNA base composition and secondary structure on complex stability.
    17. (2005). DNA bridging: a property shared among H-NSlike proteins.
    18. (1998). DNA microloops and microdomains: a general mechanism for transcription activation by torsional transmission.
    19. (2006). DNA-protein interactions and bacterial chromosome architecture.
    20. (1981). Equilibria and kinetics of Lac repressor-operator interactions by polyacrylamide gel electrophoresis.
    21. (1994). Evidence for the involvement of different genospecies of Borrelia in the clinical outcome of Lyme disease in Belgium.
    22. (2008). FHR-1, an additional human plasma protein, binds to complement regulator-acquiring surface proteins of Borrelia burgdorferi.
    23. (1997). Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi.
    24. (1987). Histonelike proteins of bacteria.
    25. (1997). Homology throughout the multiple 32-kilobase circular plasmids present in Lyme disease spirochetes.
    26. (2004). Image processing with ImageJ.
    27. (2003). Immune evasion of Borrelia burgdorferi: mapping of a complement inhibitor factor H-binding site of BbCRASP-3, a novel member of the Erp protein family.
    28. (2004). Interactive electron-density map interpretation: from INTER to O. Acta Crystallogr.
    29. (1996). Isolation of Borrelia burgdorferi genes encoding homologues of DNA-binding protein HU and ribosomal protein S20.
    30. (2008). Lyme borreliosis spirochete Erp proteins, their known host ligands, and potential roles in mammalian infection.
    31. (1999). Modulation of the nucleoid, the transcription apparatus, and the translation machinery in bacteria for stationary phase survival.
    32. (2004). Molecular characterization of Borrelia burgdorferi erp promoter/ operator elements.
    33. (1999). Molecular evidence for a new bacteriophage of Borrelia burgdorferi.
    34. (1991). MOLSCRIPT: a program to produce both detailed and schematic plots of protein structures.
    35. (1999). Natural synthesis of a DNAbinding protein from the C-terminal domain of DNA gyrase A in Borrelia burgdorferi.
    36. (1997). Raster3D photorealistic molecular graphics.
    37. (2007). Regulated synthesis of the Borrelia burgdorferi inner-membrane lipoprotein IpLA7 (P22, P22-A) during the Lyme disease spirochaete’s mammal–tick infectious cycle.
    38. (2003). Regulation of gene expression by histone-like proteins in bacteria.
    39. (2007). Regulators of expression of the oligopeptide permease A proteins of Borrelia burgdorferi.
    40. (2001). Repetition, conservation, and variation: the multiple cp32 plasmids of Borrelia species. In: Saier,M.H. and Garcı´a-Lara,J. (eds), The Spirochetes: Molecular and Cellular Biology,
    41. (1998). Rod models of DNA: sequence-dependent anisotropic elastic modelling of local bending phenomena.
    42. (2003). Sequence-specific recognition but position-dependent cleavage of two distinct telomeres by the Borrelia burgdorferi telomere resolvase,
    43. (2007). Shaping the Borrelia burgdorferi genome: crystal structure and binding properties of the DNAbending protein Hbb.
    44. (1989). Site-directed mutagensis by overlap extension using polymerase chain reaction.
    45. (2000). Site-specific DNA binding and bending by the Borrelia burgdorferi Hbb protein.
    46. (2006). The architectural role of nucleoid-associated proteins in the organization of bacterial chromatin: a molecular perspective.
    47. (1997). The bacterial nucleoid visualized by fluorescence microscopy of cells lysed within agarose: comparison of Escherichia coli and spirochetes of the genus Borrelia.
    48. (2009). The Borrelia burgdorferi infection-associated surface proteins ErpP, ErpA, and ErpC bind human plasminogen.
    49. (2009). The Borrelia burgdorferi outer-surface protein ErpX binds mammalian laminin. Microbiology,
    50. (2001). The complement Nucleic Acids Research,2009,
    51. (1981). The dimensions of DNA in solution.
    52. (2006). The Lyme disease spirochete Erp protein family: structure, function and regulation of expression.
    53. (1995). The regulation of transcription initiation by integration host factor.
    54. (1992). The role of integration host factor in gene expression in Escherichia coli.

    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.