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Expression of Immunoglobulin Gene With Classical V-(D)-J Rearrangement in Mouse Testis and Epididymis

By Jing Huang, Li Zhang, Teng Ma, Pei Zhang and Xiaoyan Qiu

Abstract

Immunoglobulin (Ig) molecules have thus far been found only to be produced by differentiated B lymphocytes. As we know, immune privilege in the normal adult mouse testis exists to make these privileged sites generally free of patrolling immune cells and Igs. However, in this study, the expression of Ig in normal adult mouse testis and epididymis was detected. First, by IHC, a strong pattern of Ig expression was detected in the spermatogenic cells of adult mouse testis at different development stages. Second, by Western blot analysis, different strong bands of Igs in mouse testicular spermatogenic cells and epididymal epithelial cell extract were recognized using specific antibodies against IgG. More importantly, by ISH and cell sorting–related RT-PCR, rearranged Ig γ chain and κ chain transcripts were expressed in testicular spermatogenic cells and epididymal epithelial cells. These results suggested that Ig in testis and epididymis was mainly produced by adult mouse testicular spermatogenic cells and epididymal epithelial cells. This manuscript contains online supplemental material at http://www.jhc.org. Please visit this article online to view these materials. (J Histochem Cytochem 57:339–349, 2009

Topics: Article
Publisher: Histochemical Society
OAI identifier: oai:pubmedcentral.nih.gov:2664983
Provided by: PubMed Central
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