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Overexpression, purification and crystallization of a thermostable DNA ligase from the archaeon Thermococcus sp. 1519

By E. Y. Bezsudnova, M. V. Kovalchuk, A. V. Mardanov, K. M. Poliakov, V. O. Popov, N. V. Ravin, K. G. Skryabin, V. A. Smagin, T. N. Stekhanova and T. V. Tikhonova

Abstract

Recombinant ATP-dependent DNA ligase from the archaeon Thermococcus sp. 1519 was overexpressed, purified and used to produce crystals that diffracted to 3.09 Å resolution

Topics: Crystallization Communications
Publisher: International Union of Crystallography
OAI identifier: oai:pubmedcentral.nih.gov:2664762
Provided by: PubMed Central
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