Skip to main content
Article thumbnail
Location of Repository

CaZF, a Plant Transcription Factor Functions through and Parallel to HOG and Calcineurin Pathways in Saccharomyces cerevisiae to Provide Osmotolerance

By Deepti Jain, Nilanjan Roy and Debasis Chattopadhyay

Abstract

Salt-sensitive yeast mutants were deployed to characterize a gene encoding a C2H2 zinc finger protein (CaZF) that is differentially expressed in a drought-tolerant variety of chickpea (Cicer arietinum) and provides salinity-tolerance in transgenic tobacco. In Saccharomyces cerevisiae most of the cellular responses to hyper-osmotic stress is regulated by two interconnected pathways involving high osmolarity glycerol mitogen-activated protein kinase (Hog1p) and Calcineurin (CAN), a Ca2+/calmodulin-regulated protein phosphatase 2B. In this study, we report that heterologous expression of CaZF provides osmotolerance in S. cerevisiae through Hog1p and Calcineurin dependent as well as independent pathways. CaZF partially suppresses salt-hypersensitive phenotypes of hog1, can and hog1can mutants and in conjunction, stimulates HOG and CAN pathway genes with subsequent accumulation of glycerol in absence of Hog1p and CAN. CaZF directly binds to stress response element (STRE) to activate STRE-containing promoter in yeast. Transactivation and salt tolerance assays of CaZF deletion mutants showed that other than the transactivation domain a C-terminal domain composed of acidic and basic amino acids is also required for its function. Altogether, results from this study suggests that CaZF is a potential plant salt-tolerance determinant and also provide evidence that in budding yeast expression of HOG and CAN pathway genes can be stimulated in absence of their regulatory enzymes to provide osmotolerance

Topics: Research Article
Publisher: Public Library of Science
OAI identifier: oai:pubmedcentral.nih.gov:2664467
Provided by: PubMed Central
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://www.pubmedcentral.nih.g... (external link)
  • Suggested articles

    Citations

    1. A (2000) Rap1p-binding sites in the saccharomyces cerevisiae GPD1 promoter are involved in its response to NaCl.
    2. (1998). A calcium sensor homolog required for plant salt tolerance.
    3. (1994). A new family of zinc finger proteins in petunia: structure, DNA sequence recognition, and floral organspecific expression.
    4. (2001). A novel coldinducible zinc finger protein from soybean, SCOF-1, enhances cold tolerance in transgenic plants.
    5. (1999). A novel mechanism of ion homeostasis and salt tolerance in yeast:
    6. (1990). A rapid and simple method for preparation of RNA from Saccharomyces cerevisiae.
    7. (1993). A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae.
    8. (1994). A two-component system that regulates an osmosensing MAP kinase cascade in yeast.
    9. (2000). Aca1 and Aca2, ATF/CREB Activators in Saccharomyces cerevisiae, Are Important for Carbon Source Utilization but Not the Response to Stress.
    10. (1995). Activation of yeast PBS2 MAPKK by MAPKKKs or by binding of an SH3-containing osmosensor.
    11. (1993). An Arabidopsis myb homolog is induced by dehydration stress and its gene product binds to the conserved MYB recognition sequence.
    12. (1993). An osmosensing signal transduction pathway in yeast.
    13. (1997). Antisense Suppression of Phospholipase D[alpha] Retards Abscisic Acid[mdash] and Ethylene-Promoted Senescence of Postharvest Arabidopsis Leaves.
    14. (2004). Arabidopsis Cys2/His2-type zinc-finger proteins function as transcription repressors under drought, cold, and high-salinity stress conditions.
    15. (1997). Calcineurin acts through the CRZ1/TCN1-encoded transcription factor to regulate gene expression in yeast.
    16. (1994). Calcineurin is essential in cyclosporin A- and FK506-sensitive yeast strains.
    17. (2003). Calcineurin signaling in Saccharomyces cerevisiae: how yeast go crazy in response to stress.
    18. (1995). Calcineurin, the Ca2+/ calmodulin-dependent protein phosphatase, is essential in yeast mutants with cell integrity defects and in mutants that lack a functional vacuolar H(+)-ATPase.
    19. (2007). Cellular processes and pathways that protect Saccharomyces cerevisiae cells against the plasma membrane-perturbing compound chitosan.
    20. (1992). Characterization of a zinc finger DNA-binding protein expressed specifically in Petunia petals and seedlings.
    21. (1998). de Larrinoa IF
    22. (2004). deacetylase to activate osmoresponsive genes.
    23. (1993). Differential expression of two genes encoding isoforms of the ATPase involved in sodium efflux in Saccharomyces cerevisiae.
    24. (2007). Dimerization and DNA-binding of ASR1, a small hydrophilic protein abundant in plant tissues suffering from water loss.
    25. (2006). Expression of CAP2, an APETALA2-Family Transcription Factor from Chickpea,
    26. (2007). Function and regulation of the Saccharomyces cerevisiae ENA sodium ATPase system.
    27. (1999). Functional characterization of ARAKIN (ATMEKK1): a possible mediator in an osmotic stress response pathway in higher plants.
    28. (1994). GPD1, which encodes glycerol-3-phosphate dehydrogenase, is essential for growth under osmotic stress in Saccharomyces cerevisiae, and its expression is regulated by the highosmolarity glycerol response pathway.
    29. (1995). Heterologous Expression of Genes in Bacterial, Fungal, Animal, and Plant Cells.
    30. (2000). High-throughput screening for proteinprotein interactions using two-hybrid assay.
    31. (2002). Hog1 kinase converts the Sko1-Cyc8-Tup1 repressor complex into an activator that recruits SAGA and SWI/SNF in response to osmotic stress.
    32. (1999). Identification and characterization of plant transporters using heterologous expression systems.
    33. (1998). Identification of a calcineurin-independent pathway required for sodium ion stress response in Saccharomyces cerevisiae.
    34. (1993). Immunosuppressants implicate protein phosphatase regulation of K+ channels in guard cells.
    35. (2004). Integration of stress responses: modulation of calcineurin signaling in Saccharomyces cerevisiae by protein kinase A.
    36. (1995). Ion Homeostasis in NaCl Stress Environments.
    37. (1996). Isolation by PCR of a cDNA clone from pea petals with similarity to petunia and wheat zinc finger proteins.
    38. (1998). MAP kinase pathways in the yeast Saccharomyces cerevisiae.
    39. (2004). MAP kinase-mediated stress relief that precedes and regulates the timing of transcriptional induction.
    40. (1994). Microbial models and salt stress tolerance in plants.
    41. (1996). Msn2p, a zinc finger DNA-binding protein, is the transcriptional activator of the multistress response in Saccharomyces cerevisiae.
    42. (1994). New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae.
    43. (2002). Novel insights into the osmotic stress response of yeast.
    44. (2003). NQK1/ NtMEK1 is a MAPKK that acts in the NPK1 MAPKKK-mediated MAPK cascade and is required for plant cytokinesis.
    45. (2003). Osmostressinduced transcription by Hot1 depends on a Hog1-mediated recruitment of the RNA Pol II.
    46. (2002). Osmotic stress signaling and osmoadaptation in yeasts.
    47. (1999). Osmotic stress-induced gene expression in Saccharomyces cerevisiae requires Msn1p and the novel nuclear factor Hot1p.
    48. (2000). Plant Cellular and Molecular Responses to High Salinity.
    49. (1982). Plant Productivity and Environment.
    50. (1998). Plants use calcium to resolve salt stress.
    51. (1993). Protein phosphatase type 2B (calcineurin)-mediated, FK506-sensitive regulation of intracellular ions in yeast is an important determinant for adaptation to high salt stress conditions.
    52. (2001). Regulation of the Sko1 transcriptional repressor by the Hog1 MAP kinase in response to osmotic stress.
    53. (2001). Repression domains of class II ERF transcriptional repressors share an essential motif for active repression.
    54. (1999). Repressors and upstream repressing sequences of the stress-regulated ENA1 gene in Saccharomyces cerevisiae: bZIP protein Sko1p confers HOG-dependent osmotic regulation.
    55. (2007). Rice shaker potassium channel OsKAT1 confers tolerance to salinity stress on yeast and rice cells.
    56. (1993). Rodriguez-Navarro A
    57. (2005). Specific binding of Autographa californica M nucleopolyhedrovirus occlusion-derived virus to midgut cells of Heliothis virescens larvae is mediated by products of pif genes Ac119 and Ac022 but not by Ac115.
    58. (1998). Stress signaling through Ca2+/calmodulin-dependent protein phosphatase calcineurin mediates salt adaptation in plants.
    59. (2000). Stress-controlled transcription factors, stress-induced genes and stress tolerance in budding yeast.
    60. (2001). Stress-induced map kinase Hog1 is part of transcription activation complexes.
    61. (1995). Studies on the transformation of intact yeast cells by the LiAc/SS-DNA/PEG procedure.
    62. (1997). Tcn1p/ Crz1p, a calcineurin-dependent transcription factor that differentially regulates gene expression in Saccharomyces cerevisiae.
    63. (1996). The Atf1 transcription factor is a target for the Sty1 stress-activated MAP kinase pathway in fission yeast.
    64. (1994). The HOG pathway controls osmotic regulation of transcription via the stress response element (STRE) of the Saccharomyces cerevisiae CTT1 gene.
    65. (1994). The protein phosphatase calcineurin is essential for NaCl tolerance of Saccharomyces cerevisiae.
    66. (1996). The Saccharomyces cerevisiae zinc finger proteins Msn2p and Msn4p are required for transcriptional induction through the stress response element (STRE).
    67. (1994). The STL1 gene of Saccharomyces cerevisiae is predicted to encode a sugar transporter-like protein.
    68. (2006). The stressactivated Hog1 kinase is a selective transcriptional elongation factor for genes responding to osmotic stress.
    69. (2000). The transcriptional response of Saccharomyces cerevisiae to osmotic shock. Hot1p and Msn2p/ Msn4p are required for the induction of subsets of high osmolarity glycerol pathway-dependent genes.
    70. (2006). The transcriptional response of the yeast Na(+)-ATPase ENA1 gene to alkaline stress involves three main signaling pathways.
    71. (2000). The transcriptional response of yeast to saline stress.
    72. (2001). Tobacco and Arabidiopsis SLT1 mediate salt tolerance of yeast.
    73. (1996). Two classes of plant cDNA clones differentially complement yeast calcineurin mutants and increase salt tolerance of wild-type yeast.
    74. (1991). Yeast has homologs (CNA1 and CNA2 gene products) of mammalian calcineurin, a calmodulin-regulated phosphoprotein phosphatase.
    75. (1998). Yeast PKA represses Msn2p/Msn4pdependent gene expression to regulate growth, stress response and glycogen accumulation.

    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.