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Nonenzymatic RNA Ligation in Water*S⃞

By Samanta Pino, Fabiana Ciciriello, Giovanna Costanzo and Ernesto Di Mauro


We describe the nonenzymatic ligation of RNA oligomers in water. Dimers and tetramers are formed in a time-, pH-, and temperature-dependent reaction. Ligation efficiency depends on oligonucleotide length and sequence and is strongly enhanced by adenine-based nucleotide cofactors. Ligation of short RNA fragments could have liberated the prebiotic polymerization systems from the thermodynamically demanding task of reaching a (pre)genetically meaningful size by stepwise addition of one precursor monomer at the time

Topics: RNA: Processing and Catalysis
Publisher: American Society for Biochemistry and Molecular Biology
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Provided by: PubMed Central
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