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Identification of a 1-epi-valienol 7-kinase activity in the producer of acarbose, Actinoplanes sp. SE50/110

By Chang-Sheng Zhang, Michael Podeschwa, Oliver Block, Hans-Josef Altenbach, Wolfgang Piepersberg and Udo F Wehmeier

Abstract

AbstractIn the biosynthesis of the C7-cyclitol moiety, valienol, of the α-glucosidase inhibitor acarbose in Actinoplanes sp. SE50/110 various cyclitol phosphates, such as 1-epi-valienol-7-phosphate, are postulated precursors. In the cell extracts of Actinoplanes SE50/110 we found a new kinase activity which specifically phosphorylates 1-epi-valienol; other C7-cyclitol analogs were only weakly or not phosphorylated. The purified product of the kinase reaction turned out to be 1-epi-valienol-7-phosphate in analyses by nuclear magnetic resonance spectroscopy. The enzyme seems not to be encoded by an acb gene and, therefore, plays a role in a salvage pathway rather than directly in the de novo biosynthesis of acarbose

Publisher: Federation of European Biochemical Societies. Published by Elsevier B.V.
Year: 2003
DOI identifier: 10.1016/S0014-5793(03)00222-9
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