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What is the Most Sensitive Measure of Water Maze Probe Test Performance?

By Hamid R. Maei, Kirill Zaslavsky, Cátia M. Teixeira and Paul W. Frankland

Abstract

The water maze is commonly used to assay spatial cognition, or, more generally, learning and memory in experimental rodent models. In the water maze, mice or rats are trained to navigate to a platform located below the water's surface. Spatial learning is then typically assessed in a probe test, where the platform is removed from the pool and the mouse or rat is allowed to search for it. Performance in the probe test may then be evaluated using either occupancy-based (percent time in a virtual quadrant [Q] or zone [Z] centered on former platform location), error-based (mean proximity to former platform location [P]) or counting-based (platform crossings [X]) measures. While these measures differ in their popularity, whether they differ in their ability to detect group differences is not known. To address this question we compiled five separate databases, containing more than 1600 mouse probe tests. Random selection of individual trials from respective databases then allowed us to simulate experiments with varying sample and effect sizes. Using this Monte Carlo-based method, we found that the P measure consistently outperformed the Q, Z and X measures in its ability to detect group differences. This was the case regardless of sample or effect size, and using both parametric and non-parametric statistical analyses. The relative superiority of P over other commonly used measures suggests that it is the most appropriate measure to employ in both low- and high-throughput water maze screens

Topics: Neuroscience
Publisher: Frontiers Research Foundation
OAI identifier: oai:pubmedcentral.nih.gov:2659169
Provided by: PubMed Central
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    Citations

    1. (2000). A learning defi cit related to age and beta-amyloid plaques in a mouse model of Alzheimer’s disease.
    2. (2007). Analysis of the trajectory of Drosophila melanogaster in a circular open fi eld arena.
    3. (1984). Development of a water-maze procedure for studying spatial learning in the rat.
    4. (1998). Distributed encoding and retrieval of spatial memory in the hippocampus.
    5. (2003). Extinction, renewal, and spontaneous recovery of a spatial preference in the water maze.
    6. (2004). Forgetting, reminding, and remembering: the retrieval of lost spatial memory.
    7. (1998). Genetically modifi ed mice and cognition.
    8. (1997). Hippocampal lesions cause learning deficits in inbred mice in the Morris water maze and conditioned-fear task.
    9. (2007). Imaging activation of adult-generated granule cells in spatial memory.
    10. (1992). Impaired spatial learning in alpha-calcium-calmodulin kinase II mutant mice.
    11. (1999). Impaired synaptic plasticity and learning in aged amyloid precursor protein transgenic mice.
    12. (2004). Memory reconsolidation and extinction have distinct temporal and Frontiers in Integrative Neuroscience www.frontiersin.org
    13. (2006). Morris water maze: procedures for assessing spatial and related forms of learning and memory.
    14. (2008). Mouse cognition-related behavior in the open-fi eld: emergence of places of attraction.
    15. (2009). paper pending published: 12
    16. (1982). Place navigation impaired in rats with hippocampal lesions.
    17. (1992). Power of the independent samples t test under a prevalent psychometric measure distribution.
    18. (2007). Preferential incorporation of adult-generated granule cells into spatial memory networks in the dentate gyrus.
    19. (1999). Reversible neural inactivation reveals hippocampal participation in several memory processes.
    20. (1986). Selective impairment of learning and blockade of long-term potentiation by an Nmethyl-d-aspartate receptor antagonist, AP5.
    21. (1993). Severity of spatial learning impairment in aging: development of a learning index for performance in the Morris water maze.
    22. (1993). Spatial learning impairment parallels the magnitude of dorsal hippocampal lesions, but is hardly present following ventral lesions.
    23. (1981). Spatial localisation does not depend on the presence of local cues.
    24. (1998). Spatial memory and learning in transgenic mice: fact or artifact?
    25. (2004). Survey of embryonic stem cell line source strains in the water maze reveals superior reversal learning of 129S6/SvEvTac mice.
    26. (1996). The essential role of hippocampal CA1 NMDA receptor-dependent synaptic plasticity in spatial memory.
    27. (2009). The precision of remote context memories does not require the hippocampus.
    28. (2009). What is the most sensitive measure of water maze probe test performance?

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