Skip to main content
Article thumbnail
Location of Repository

Potato (Solanum tuberosum L.) tuber ageing induces changes in the proteome and antioxidants associated with the sprouting pattern

By Pierre Delaplace, Marie-Laure Fauconnier, Kjell Sergeant, Jean-François Dierick, Mouhssin Oufir, Froukje van der Wal, Antoine H. P. America, Jenny Renaut, Jean-François Hausman and Patrick du Jardin

Abstract

During post-harvest storage, potato tubers age as they undergo an evolution of their physiological state influencing their sprouting pattern. In the present study, physiological and biochemical approaches were combined to provide new insights on potato (Solanum tuberosum L. cv. Désirée) tuber ageing. An increase in the physiological age index (PAI) value from 0.14 to 0.83 occurred during storage at 4 °C over 270 d. Using this reference frame, a proteomic approach was followed based on two-dimensional electrophoresis. In the experimental conditions of this study, a marked proteolysis of patatin occurred after the PAI reached a value of 0.6. In parallel, several glycolytic enzymes were up-regulated and cellular components influencing protein conformation and the response to stress were altered. The equilibrium between the 20S and 26S forms of the proteasome was modified, the 20S form that recycles oxidized proteins being up-regulated. Two proteins belonging to the cytoskeleton were also differentially expressed during ageing. As most of these changes are also observed in an oxidative stress context, an approach focused on antioxidant compounds and enzymes as well as oxidative damage on polyunsaturated fatty acids and proteins was conducted. All the changes observed during ageing seemed to allow the potato tubers to maintain their radical scavenging activity until the end of the storage period as no accumulation of oxidative damage was observed. These data are interpreted considering the impact of reactive oxygen species on the development and the behaviour of other plant systems undergoing ageing or senescence processes

Topics: Research Papers
Publisher: Oxford University Press
OAI identifier: oai:pubmedcentral.nih.gov:2657538
Provided by: PubMed Central
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://www.pubmedcentral.nih.g... (external link)
  • Suggested articles

    Citations

    1. (2007). A DiGE analysis of developing poplar leaves subjected to ozone reveals major changes in carbon metabolism.
    2. (1997). A peroxidase/phenolics/acorbate system can scavenge hydrogen peroxide in plant cells.
    3. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding.
    4. (1956). A simple ultraviolet spectrophotometric method for determination of protein.
    5. (2002). Accumulation of plant small heat-stress proteins in storage organs.
    6. (2006). Actin dynamics: old friends with new stories.
    7. (1999). Age-induced protein modifications and increased proteolysis in potato seed-tubers.
    8. (1956). Aging: a theory based on free radical and radiation chemistry.
    9. (2000). Antioxidant content of whole grain breakfast cereals, fruits and vegetables.
    10. (2007). Antioxidant profiling of native Andean potato cultivars (Solanum tuberosum L.) reveals tubers with high levels of b-carotene, a-tocopherol, chlorogenic acid and patanin.
    11. (1996). Antioxidant responses to drought in sunflower and sorghum seedlings.
    12. (2005). Antioxidants and reactive oxygen species in plants.
    13. (1998). Ascorbate peroxidase and catalase cooperate for protection against hydrogen peroxide generated in potato tubers during low-temperature storage.
    14. (2005). Ascorbate peroxidase.
    15. (2005). Ascorbate, tocopherol and carotenoids: metabolism, pathway engineering and functions.
    16. (1997). Aspects of amino acid metabolism in stored potato tubers (cv. Pentland Dell).
    17. (2004). Carotenogenesis during tuber development and storage in potato.
    18. (1985). Catalase activity. In:
    19. (2007). Catalase inhibition accelerates dormancy release and sprouting in potato (Solanum tuberosum L.) tubers.
    20. (2005). Catalases in plants: molecular and functional properties and role in stress defence.
    21. (1993). Changes in lipid peroxidation and lipolytic and free-radical scavenging enzyme activities during aging and sprouting of potato (Solanum tuberosum) seed-tubers.
    22. (2008). Changes in oxylipin synthesis after Phytophthora infestans infection of potato leaves do not correlate with resistance.
    23. (1999). Changes in the potato (Solanum tubersoum L.) tuber at the onset of dormancy and during storage at 23
    24. (1994). Changes in the two-dimensional protein pattern and in gene expression during the sink-to-source transition of potato tubers.
    25. (1994). Characterization of the cDNA clones of 2 beta-tubulin genes and their expression in the potato plant (Solanum tuberosum L.).
    26. (1996). Characterization of three potato lipoxygenases with distinct enzymatic activities and different organ-specific and wound-regulated expression patterns.
    27. (2002). Class I heat-shock protein gives thermotolerance in tobacco.
    28. (2001). Clinica Chimica Acta 329, 23–38. 1286 | Delaplace et al.Davies KJA.
    29. (2008). Comparative proteomics of tuber induction, development and maturation reveal the complexity of tuberization process in potato (Solanum tuberosum L.).
    30. (2004). Dehydroascorbate influences the plant cell cycle through a glutathione-independent reduction mechanism.
    31. (2008). Dormancy in potato tuber meristems: chemically induced cessation in dormancy matches the natural process based on transcript profiles.
    32. (2008). e ´thodes de mesure de l’a ˆge physiologique des tubercules semences de pomme de terre (Solanum tuberosum L.
    33. (1986). EAPR working group physiological age of the potato.
    34. (1985). Effects of calcium on sprout growth of ten potato cultivars.
    35. (1995). Environment and plant metabolism: flexibility and acclimation.
    36. (1999). Evolving concepts in plant glycolysis: two centuries of progress.
    37. (1994). Extraction and determination of ascorbate and dehydroascorbate from plant tissue.
    38. (1996). Free radicals: a practical approach.
    39. (1981). Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts.
    40. (2002). Impairment of proteasome structure and function in aging.
    41. (2001). In situ kinetic analysis of glyoxalase I and glyoxalase II in Potato tuber ageing during post-harvest storage | 1287Saccharomyces cerevisiae.
    42. (1973). Influence of temperature during the formation of tubers in relation with their incubation state (physiological age) and seed value.
    43. (2006). Intranuclear accumulation of plant tubulin in response to low temperature.
    44. (2005). Isolation and expression of protein disulfide isomerase cDNA from sweet potato (Ipomoea batatas [L.] Lam ‘Tainong 57’) storage roots.
    45. (2007). Leaf senescence and activities of the antioxidant enzymes.
    46. (1981). Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase.
    47. (2005). Les compose ´s phe ´noliques des ve ´ge ´taux. Un exemple de me ´tabolites secondaires d’importance e ´conomique. Lausanne: Presses polytechniques et universitaires romandes.
    48. (2003). Lipid peroxidation during the hypersensitive response in potato in the absence of 9-lipoxygenases.
    49. (2002). Lipoxygenase pathway and membrane permeability and composition during storage of potato tubers (Solanum tuberosulm L. cv. Bintje and De ´sire ´e) in different conditions.
    50. (2006). Lipoxygenases: occurrence, functions and catalysis.
    51. (2006). Metabolic and proteomic markers for oxidative stress. New tools for reactive oxygen species research.
    52. (2005). Methylglyoxal levels in plants under salinity stress are dependent on glyoxalase I and glutathione.
    53. (1998). Molecular organization of the 20S proteasome gene family from Arabidopsis thaliana.
    54. (2008). Non-redundant functions of sHSP-CIs in acquired thermotolerance and their role in early seed development in Arabidopsis.
    55. (2002). Oxidative metabolism and the physiological age of seed potatoes are affected by increased a-linolenate content.
    56. (2007). Oxidative stress and leaf senescence. In:
    57. (1996). Oxidative stress results in increased sinks for metabolic energy during aging and sprouting of potato seed-tubers.
    58. (2001). Oxidative stress, growth factor production and budding in potato tuber during cold storage.
    59. (2008). Oxylipin profile and antioxidant status of potato tubers during extended storage at room temperature.
    60. (2002). Oxylipin profiling in pathogen infected potato leaves.
    61. (2001). Physiological age index: a new, simple and reliable index to assess the physiological age of seed potato tubers based on the haulm killing date and length of the incubation period.
    62. (2000). Physiological ageing of potato tubers: a review.
    63. (1989). Post-harvest physiology. In:
    64. (2008). Potato (Solanum tuberosum L.) tuber physiological age index is a valid reference frame in postharvest ageing studies.
    65. (2006). Potato tuber proteomics: comparison of two complementary extraction methods designed for 2-DE of acidic proteins.
    66. (2003). Protein carbonyl groups as biomarkers of oxidative stress.
    67. (1995). Protein changes in Solanum tuberosum during storage and dormancy breaking of in vitro microtubers.
    68. (2007). Protein disulfide isomerase family proteins involved in soybean protein biogenesis.
    69. (2006). Protein disulfide isomerase: the structure of oxidative folding.
    70. (2006). Proteomic analysis of the potato tuber life cycle.
    71. (2006). Reactive species and antioxidants. Redox biology is a fundamental theme of aerobic life.
    72. (2005). Redox regulation: a broadening horizon.
    73. (2002). Role of oxidative stress and protein oxidation in the aging process.
    74. (2002). Small heat shock proteins and stress tolerance in plants.
    75. (2005). Small heat shock proteins are differentially regulated during pollen development and following heat stress in tobacco.
    76. (1964). Spectrophometric method for determination of lipoxidase activity.
    77. (1990). Superoxide dismutase, catalase, and a-tocopherol content of stored potato tubers.
    78. (1997). The ascorbate system and lipid peroxidation in stored potato (Solanum tuberosum L.) tubers.
    79. (1995). The expression of a small heat shock gene is activated during induction of tobacco pollen embryogenesis by starvation.
    80. (1999). The redox state of the ascorbate–dehydroascorbate pair as a specific sensor of cell division in tobacco TBY2 cells.
    81. (2003). The relationship between changes in the cell wall, lipid peroxidation, proliferation, senescence and cell death.
    82. (2004). The ubiquitin 26S proteasome proteolytic pathway.
    83. (2007). Transcriptional profiling of Arabidopsis heat shock proteins and transcription factors reveals extensive overlap between heat and non-heat stress response pathways.

    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.