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Menthol Enhances an Antiproliferative Activity of 1α,25-Dihydroxyvitamin D3 in LNCaP Cells

By Eun-Jung Park, Su-Hwa Kim, Byung-Joo Kim, Sung-Young Kim, Insuk So and Ju-Hong Jeon

Abstract

1α,25-dihydroxyvitamin D3 [1α,25(OH)2D3], the most active form of vitamin D3, and its analogues have therapeutic benefits for prostate cancer treatment. However, the development of hypercalcemia is an obstacle to clinical applications of 1α,25(OH)2D3 for cancer therapy. In this study, we provide evidence that menthol, a key component of peppermint oil, increases an anti-proliferation activity of 1α,25(OH)2D3 in LNCaP prostate cancer cells. We found that menthol per se does not exhibit antiproliferative activity, but it is able to enhance 1α,25(OH)2D3-mediated growth inhibition in LNCaP cells. Fluorometric assays using Fura-2 showed that 1α,25(OH)2D3 does not induce acute Ca2+ response, whereas menthol evokes an increase in [Ca2+]i, which suggests that cross-talks of menthol-induced Ca2+ signaling with 1α,25(OH)2D3-mediated growth inhibition pathways. In addition, Western blot analysis revealed that 1α,25(OH)2D3 and menthol cooperatively modulate the expression of bcl-2 and p21 which provides the insight into the molecular mechanisms underlying the enhanced 1α,25(OH)2D3-mediated growth inhibition by menthol. Thus, our findings suggest that menthol may be a useful natural compound to enhance therapeutic effects of 1α,25(OH)2D3

Topics: Original Article
Publisher: the Society for Free Radical Research Japan
OAI identifier: oai:pubmedcentral.nih.gov:2654468
Provided by: PubMed Central
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    Citations

    1. (2006). A review of the bioactivity and potential health benefits of peppermint tea (Mentha piperita L.).
    2. (2004). Antitumor activity of calcitriol: pre-clinical and clinical studies.
    3. (2001). Calcitriol (1,25-dihydroxycholecalciferol) enhances paclitaxel antitumor activity in vitro and in vivo and accelerates paclitaxelinduced apoptosis.
    4. (2007). Calcium and cancer: targeting Ca2+ transport.
    5. (2006). Cancer statistics
    6. (2004). Evidence that TRPM8 is an androgen-dependent Ca2+ channel required for the survival of prostate cancer cells.
    7. (2007). Inflammation in prostate carcinogenesis.
    8. (2005). Largescale in silico and microarray-based identification of direct 1,25-dihydroxyvitamin D3 target genes.
    9. (2007). Marked disturbance of calcium homeostasis in mice with targeted disruption of the Trpv6 calcium channel
    10. (2007). Menthol: a refreshing look at this ancient compound.
    11. (2006). Minireview: vitamin D receptor: new assignments for an already busy receptor.
    12. (2001). The development of androgenindependent prostate cancer.
    13. (2006). The human transient receptor potential vanilloid type 6 distal promoter contains multiple vitamin D receptor binding sites that mediate activation by 1,25-dihydroxyvitamin D3 in intestinal cells.
    14. (2007). TRPV6 channel controls prostate cancer cell proliferationE.-J.
    15. (2004). Vitamin D and breast cancer: insights from animal models.
    16. (2007). Vitamin D signalling pathways in cancer, potential for anticancer therapeutics.

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