Skip to main content
Article thumbnail
Location of Repository

One-dimensional topography underlies three-dimensional fibrillar cell migration

By Andrew D. Doyle, Francis W. Wang, Kazue Matsumoto and Kenneth M. Yamada

Abstract

Current concepts of cell migration were established in regular two-dimensional (2D) cell culture, but the roles of topography are poorly understood for cells migrating in an oriented 3D fibrillar extracellular matrix (ECM). We use a novel micropatterning technique termed microphotopatterning (μPP) to identify functions for 1D fibrillar patterns in 3D cell migration. In striking contrast to 2D, cell migration in both 1D and 3D is rapid, uniaxial, independent of ECM ligand density, and dependent on myosin II contractility and microtubules (MTs). 1D and 3D migration are also characterized by an anterior MT bundle with a posterior centrosome. We propose that cells migrate rapidly through 3D fibrillar matrices by a 1D migratory mechanism not mimicked by 2D matrices

Topics: Research Articles
Publisher: The Rockefeller University Press
OAI identifier: oai:pubmedcentral.nih.gov:2654121
Provided by: PubMed Central
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://www.pubmedcentral.nih.g... (external link)
  • Suggested articles

    Citations

    1. (2005). A Rac switch regulates random versus directionally persistent cell migration.
    2. (1997). Cell locomotion and focal adhesions are regulated by substrate fl exibility.
    3. (1998). Cell locomotion and focal adhesions are regulated by the mechanical properties of the substrate.
    4. (2005). Cell migration in 3D matrix.
    5. (2003). Cell migration: integrating signals from front to back.
    6. (2008). Contact guidance mediated three-dimensional cell migration is regulated by Rho/ ROCK-dependent matrix reorganization.
    7. (1996). Controlling cell attachment on contoured surfaces with self-assembled monolayers of alkanethiolates on gold.
    8. (1984). Experimental manipulation of a contact guidance system in amphibian gastrulation by mechanical tension.
    9. (2008). In migrating cells, the Golgi complex and the position of the centrosome depend on geometrical constraints of the substratum.
    10. (1979). Induction of spreading during fi broblast movement.
    11. (2001). Integrin-mediated activation of Cdc42 controls cell polarity in migrating astrocytes through PKCzeta.
    12. (2006). Matrix elasticity directs stem cell lineage specifi cation.
    13. (1993). Maximal migration of human smooth muscle cells on fi bronectin and type IV collagen occurs at an intermediate attachment strength.
    14. (1981). Mechanism of retraction of the trailing edge during fi broblast movement.
    15. (2006). Migration of tumor cells in 3D matrices is governed by matrix stiffness along with cell-matrix adhesion and proteolysis.
    16. (2007). Modeling tissue morphogenesis and cancer
    17. (2007). Myosin IIA regulates cell motility and actomyosinmicrotubule crosstalk.
    18. (2003). Nanofabrication for micropatterned cell arrays by combining electron beam-irradiated polymer grafting and localized laser ablation.
    19. (2006). Of extracellular matrix, scaffolds, and signaling: tissue architecture regulates development, homeostasis, and cancer.
    20. (2006). Probing the microenvironment of mammary tumors using multiphoton microscopy.
    21. (2008). Rapid leukocyte migration by integrin-independent fl owing and squeezing.
    22. (1988). Selective stabilization of microtubules oriented toward the direction of cell migration.
    23. (1999). Selective stabilization of microtubules within the proximal region of developing axonal neurites.
    24. (1952). Shape and movement of mesenchyme cells as functions of the physical structure of the medium: contributions to a quantitative morphology.
    25. (2006). Spatiotemporal feedback between actomyosin and focal-adhesion systems optimizes rapid cell migration.
    26. (2001). Taking cellmatrix adhesions to the third dimension. Science .

    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.