Skip to main content
Article thumbnail
Location of Repository

Overexpressed vs mutated Kras in murine fibroblasts: a molecular phenotyping study

By M Horsch, C V Recktenwald, S Schädler, M Hrabé de Angelis, B Seliger and J Beckers


Ras acts in signalling pathways regulating the activity of multiple cellular functions including cell proliferation, differentiation, and apoptosis. Amino-acid exchanges at position 12, 13, or 61 of the Kras gene convert the proto-oncogene into an activated oncogene. Until now, a direct comparison of genome-wide expression profiling studies of Kras overexpression and different Kras mutant forms in a single assay system has not been carried out. In our study, we focused on the direct comparison of global gene expression effects caused by mutations in codon 12 or 13 of the Kras gene and Kras overexpression in murine fibroblasts. We determined Kras cellular mRNA, Ras protein and activated Ras protein levels. Further, we compared our data to the proteome analysis of the same transfected cell lines. Both overexpression and mutations of Kras lead to common altered gene expression patterns. Only two genes, Lox and Col1a1, were reversely regulated in the Kras transfectants. They may contribute to the higher aggressiveness of the Kras codon 12 mutation in tumour progression. The functional annotation of differentially expressed genes revealed a high frequency of proteins involved in tumour growth and angiogenesis. These data further support the important role of these genes in tumour-associated angiogenesis

Topics: Molecular Diagnostics
Publisher: Nature Publishing Group
OAI identifier:
Provided by: PubMed Central

Suggested articles


  1. (2007). Breast cancer molecular signatures as determined by SAGE: correlation with lymph node status.
  2. (2007). Influence of Ki-ras-driven oncogenic transformation on the protein network of murine fibroblasts.
  3. (2008). Regulating the angiogenic balance in tissues.
  4. (1994). SPARC is a source of copper-binding peptides that stimulate angiogenesis. J Cell Biol 125: 929–943 Marshall CJ

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.