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New cryptosporidium genotypes in HIV-infected persons.

By N. J. Pieniazek, F. J. Bornay-Llinares, S. B. Slemenda, A. J. da Silva, I. N. Moura, M. J. Arrowood, O. Ditrich and D. G. Addiss


Using DNA sequencing and phylogenetic analysis, we identified four distinct Cryptosporidium genotypes in HIV-infected patients: genotype 1 (human), genotype 2 (bovine) Cryptosporidium parvum, a genotype identical to C. felis, and one identical to a Cryptosporidium sp. isolate from a dog. This is the first identification of human infection with the latter two genotypes

Topics: Research Article
Publisher: Centers for Disease Control
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Provided by: PubMed Central

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  1. (1991). A technique for typing Cryptosporidium isolates.
  2. (1991). A workbench for multiple alignment construction and analysis.
  3. Cryptosporidiosis in humans: review of recent epidemiologic studies.
  4. Cryptosporidium wrairi sp. n. from the guinea pig Cavia porcellus, with an emendation of the genus.
  5. (1999). Declining CD4+ T-lymphocyte counts are associated with increased risk of enteric parasitosis and chronic diarrhea: results of a 3-year longitudinal study. J Acquir Immune Defic Syndr Hum Retrovirol
  6. (1995). Development of a PCR protocol for sensitive detection of Cryptosporidium oocysts in water samples.
  7. (1997). Differentiation between human and animal isolates of Cryptosporidium parvum using rDNA sequencing and direct PCR analysis.
  8. (1995). Differentiation between human and animal strains of Cryptosporidium parvum using isoenzyme typing. Parasitology
  9. (1998). Differentiation of phylogenetically related slowly growing mycobacteria based on 16S-23S rRNA gene internal transcribed spacer sequences.
  10. (1993). DNA probe hybridization and PCR detection of Cryptosporidium parvum compared to immunofluorescence assay.
  11. (1992). Evaluation of a new monoclonal antibody combination reagent for the direct fluorescent detection of Giardia cysts and Cryptosporidium oocysts in human fecal specimens.
  12. (1999). Fast and reliable extraction of protozoan parasite DNA from fecal specimens. Molecular Diagnosis. In press
  13. (1998). Genetic heterogeneity and PCR detection of Cryptosporidium parvum.
  14. (1994). Genetic markers differentiate C. parvum isolates.
  15. (1997). Genetic polymorphism among Cryptosporidium parvum isolates: evidence of two distinct human transmission cycles. Emerg Infect Dis
  16. (1998). Genotypic and phenotypic characterization of Cryptosporidium parvum isolates from people with AIDS.
  17. (1996). Genotyping human and bovine isolates of Cryptosporidium parvum by polymerase chain reaction-restriction fragment length polymorphism analysis of a repetitive DNA sequence.
  18. (1994). Geographical variation in 18S rRNA gene sequence of Cryptosporidium parvum.
  19. (1999). Identification of Cryptosporidium felis in a cow by morphologic and molecular methods.
  20. (1996). Identification of genetic heterogeneity in the Cryptosporidium parvum ribosomal repeat.
  21. (1997). Laboratory models of cryptosporidiosis.
  22. (1982). Laboratory procedures for the diagnosis of intestinal parasites. 3rd ed. Atlanta: Centers for Disease Control;
  23. (1995). Massive outbreak of waterborne Cryptosporidium infection in Milwaukee, Wisconsin: recurrence of illness and risk of secondary transmission. Clin Infect Dis
  24. (1998). Molecular characterization of Cryptosporidium from various hosts. Parasitology
  25. (1995). Molecular characterization of Cryptosporidium isolates from humans and other animals using random amplified polymorphic DNA analysis.
  26. (1998). Morphological and genetic characterization of Cryptosporidium oocysts from domestic cats. Vet Parasitol
  27. (1998). Natural history and biology of Cryptosporidium parvum.
  28. (1992). PCR cloning and nucleotide sequence determination of the 18S rRNA genes and internal transcribed spacer 1 of the protozoan parasites Cryptosporidium parvum and Cryptosporidium muris.
  29. (1997). PCR-RFLP analysis of the Cryptosporidium oocyst wall protein (COWP) gene discriminates between C. wrairi and C. parvum, and between C. parvum isolates of human and animal origin.
  30. (1989). PHYLIPphylogeny inference package.
  31. (1998). Randomly amplified polymorphic DNA PCR analysis of bovine Cryptosporidium parvum strains isolated from the watershed of the Red River of the North. Appl Environ Microbiol
  32. (1997). Ribosomal RNA gene organization in Cryptosporidium parvum. Mol Biochem Parasitol
  33. (1994). Similarities and differences between DNA of Cryptosporidium parvum and C. wrairi detected by the polymerase chain reaction. Folia Parasitol
  34. (1998). The epidemiology of cryptosporidiosis: application of experimental sub-typing and antibody detection systems to the investigation of water-borne outbreaks. Folia Parasitol
  35. The general biology of Cryptosporidium. In:

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