Article thumbnail

Endophilin Is Required for Synaptic Vesicle Endocytosis by Localizing Synaptojanin

By Kimberly R Schuske, Janet E Richmond, Dawn Signor Matthies, Warren S Davis, Steffen Runz, Daniel A Rube, Alexander M van der Bliek and Erik M Jorgensen

Abstract

AbstractEndophilin is a membrane-associated protein required for endocytosis of synaptic vesicles. Two models have been proposed for endophilin: that it alters lipid composition in order to shape membranes during endocytosis, or that it binds the polyphosphoinositide phosphatase synaptojanin and recruits this phosphatase to membranes. In this study, we demonstrate that the unc-57 gene encodes the Caenorhabditis elegans ortholog of endophilin A. We demonstrate that endophilin is required in C. elegans for synaptic vesicle recycling. Furthermore, the defects observed in endophilin mutants closely resemble those observed in synaptojanin mutants. The electrophysiological phenotype of endophilin and synaptojanin double mutants are virtually identical to the single mutants, demonstrating that endophilin and synaptojanin function in the same pathway. Finally, endophilin is required to stabilize expression of synaptojanin at the synapse. These data suggest that endophilin is an adaptor protein required to localize and stabilize synaptojanin at membranes during synaptic vesicle recycling

Publisher: Cell Press. Published by Elsevier Inc.
Year: 2003
DOI identifier: 10.1016/S0896-6273(03)00667-6
OAI identifier:

Suggested articles


To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.