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Isolation of the intermediate filament protein vimentin by chromatofocusing

By Hans Bloemendal, Mia Willemsen, Gerrit Groenewoud and Paul Oomen

Abstract

AbstractA novel, simple and relatively rapid method is described for the isolation of the intermediate-sized filament protein vimentin from eye lens tissue. Chromatofocusing is applied as the sole purification step. The apparent isoelectric point of the protein in 6 M urea and at 22°C is 4.9. Electrophoretic mobility on one- and two-dimensional polyacrylamide gels, solubility in 6 M urea and amino acid composition were used for identificatio

Publisher: Published by Elsevier B.V.
Year: 1985
DOI identifier: 10.1016/0014-5793(85)81067-X
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