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Comparison and optimization of methods for the simultaneous extraction of DNA, RNA, proteins, and metabolites

By Fränze Vorreiter, Silke Richter, Michel Peter, Sven Baumann, Martin von Bergen and Janina M. Tomm

Abstract

AbstractThe challenge of performing a time-resolved comprehensive analysis of molecular systems has led to the quest to optimize extraction methods. When the size of a biological sample is limited, there is demand for the simultaneous extraction of molecules representing the four areas of “omics”: genomics, transcriptomics, proteomics, and metabolomics. Here we optimized a protocol for the simultaneous extraction of DNA, RNA, proteins, and metabolites and compared it with two existing protocols. Our optimization comprised the addition of a methanol/chloroform metabolite purification before the separation of DNA/RNA and proteins. Extracted DNA, RNA, proteins, and metabolites were quantitatively and/or qualitatively analyzed. Of the three methods, only the newly developed protocol yielded all biomolecule classes of adequate quantity and quality

Publisher: The Author(s). Published by Elsevier Inc.
Year: 2016
DOI identifier: 10.1016/j.ab.2016.05.011
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