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Molecular Method for Discrimination between Francisella tularensis and Francisella-Like Endosymbionts▿

By Raquel Escudero, A. Toledo, Horacio Gil, Katarina Kovácsová, Manuela Rodríguez-Vargas, Isabel Jado, Cristina García-Amil, Bruno Lobo, Mangesh Bhide and Pedro Anda

Abstract

Environmental studies on the distribution of Francisella spp. are hampered by the frequency of Francisella-like endosymbionts that can produce a misleading positive result. A new, efficient molecular method for detection of Francisella tularensis and its discrimination from Francisella-like endosymbionts, as well as two variants associated with human disease (unusual F. tularensis strain FnSp1 and F. tularensis subsp. novicida-like strain 3523), is described. The method is highly specific and sensitive, detecting up to one plasmid copy or 10 genome equivalents

Topics: Bacteriology
Publisher: American Society for Microbiology (ASM)
OAI identifier: oai:pubmedcentral.nih.gov:2546735
Provided by: PubMed Central
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