Article thumbnail
Location of Repository

Imaging and characterizing influenza A virus mRNA transport in living cells

By Wei Wang, Zong-Qiang Cui, Han Han, Zhi-Ping Zhang, Hong-Ping Wei, Ya-Feng Zhou, Ze Chen and Xian-En Zhang

Abstract

The mechanisms of influenza A virus mRNA intracellular transport are still not clearly understood. Here, we visualized the distribution and transport of influenza A virus mRNA in living cells using molecular beacon (MB) technology. Confocal-FRAP measurements determined that the transport of influenza A virus intronless mRNA, in both nucleus and cytoplasm, was energy dependent, being similar to that of Poly(A)+ RNA. Drug inhibition studies in living cells revealed that the export of influenza A virus mRNA is independent of the CRM1 pathway, while the function of RNA polymerase II (RNAP-II) may be needed. In addition, viral NS1 protein and cellular TAP protein were found associated with influenza A virus mRNA in the cell nucleus. These findings characterize influenza A virus mRNA transport in living cells and suggest that influenza A virus mRNA may be exported from the nucleus by the cellular TAP/p15 pathway with NS1 protein and RNAP-II participation

Topics: RNA
Publisher: Oxford University Press
OAI identifier: oai:pubmedcentral.nih.gov:2528172
Provided by: PubMed Central

Suggested articles

Citations

  1. (2002). A conserved mRNA export machinery coupled to pre-mRNA splicing.
  2. (2004). CRM1-dependent, but not ARE-mediated, nuclear export of IFNa1mRNA.
  3. (2001). Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O.
  4. (2004). Dual FRET molecular beacons for mRNA detection in living cells.
  5. (2005). Dynamic sorting of nuclear components into distinct nucleolar caps during transcriptional inhibition.
  6. (2007). Dynamics of filamentous viral RNPs prior to egress.
  7. (2004). Dynamics of Single mRNPs in Nuclei of Living Cells.
  8. (1994). Evidence that HIV-1 Rev directly promotes the nuclear export of unspliced RNA.
  9. (1992). Export of mRNA from microinjected nuclei of Xenopus laevis oocytes.
  10. (2000). Herpesvirus mRNAs are sorted for export via CRM1-dependent and -independent pathways.
  11. (2000). High mobility of proteins in the mammalian cell nucleus.
  12. (1991). HIV-1 structural gene expression requires the binding of multiple rev monomers to the viral RRE: implications for latency.
  13. (2004). Hypophosphorylated ASF/SF2 binds TAP and is present in messenger ribonucleoproteins.
  14. (2002). ICP27 Interacts with the RNA export factor Aly/REF to direct herpes simplex virus type 1 intronless mRNAs to the TAP export pathway.
  15. (1999). Identification of novel import and export signals of human TAP, the protein that binds to the constitutive transport element of the type D retrovirus mRNAs.
  16. (2004). Imaging Native b-Actin mRNA in Motile Fibroblasts.
  17. (2006). Immunoprecipitation of mRNAprotein complexes.
  18. (1998). In situ hybridization of mammalian cells
  19. (1999). Influenza A virus NS1 protein targets poly(A)-binding protein II of the cellular 30-end processing machinery.
  20. (1994). Influenza virus NS1 protein inhibits pre-mRNA splicing and blocks mRNA nucleocytoplasmic transport.
  21. (1997). Influenza virus NS1 protein interacts with viral transcription– replication complexes in vivo.
  22. (1991). Intracellular distribution of microinjected antisense oligonucleotide.
  23. (1999). Involvement of CRM1, a nuclear export receptor, in mRNA export in mammalian cells and fission yeast.
  24. (2006). Live-cell characterization and analysis of a clinical isolate of bovine respiratory syncytial virus, using molecular beacons.
  25. (2005). Mechanism of mRNA transport in the nucleus.
  26. (1999). Movement of nuclear poly(A) RNA throughout the interchromatin space in living cells.
  27. (2007). Mutation analysis of a recombinant NS replicon shows that influenza virus NS1 protein blocks the splicing and nucleo-cytoplasmic transport of its own viral mRNA.
  28. (2007). Nuclear Export of Influenza A virus mRNAs Requires Ongoing RNA Polymerase II Activity.
  29. (2000). Nuclear RNA export pathways.
  30. (2003). Nuclear RNA export.
  31. (2003). PABP1 and eIF4GI associate with influenza virus NS1 protein in viral mRNA translation initiation complexes.
  32. (2004). Peptide-linked molecular beacons for efficient delivery and rapid mRNA detection in living cells.
  33. (2001). Phosphoramidate oligonucleotides as potent antisense molecules in cells and in vivo.
  34. (2004). Poly(A)+ RNAs roam the cell nucleus and pass through speckle domains in transcriptionally active and inactive cells.
  35. (1991). Regulated M1 mRNA splicing in influenza virus-infected cells.
  36. (2000). Selective nuclear export of viral mRNAs in influenza-virus-infected cells.
  37. (1998). TAP, the human homologue of Mex67p, mediates CTE-dependent RNA export from the nucleus.
  38. (2000). The C-terminal domain of TAP interacts with the nuclear pore complex and promotes export of specific CTE-bearing RNA substrates.
  39. (1999). The human tap protein is a nuclear mRNA export factor that contains novel RNA-binding and nucleocytoplasmic transport sequences.
  40. (1994). The influenza virus NS1 protein is a poly(A)-binding protein that inhibits nuclear export of mRNAs containing poly(A).
  41. (2002). The influenza virus nucleoprotein: a multifunctional RNA-binding protein pivotal to virus replication.
  42. (1999). The Mex-67p-mediated nuclear mRNA export pathway is conserved from yeast to human.
  43. (1999). The NES-Crm1 export pathway is not a major mRNA export route in Saccharomyces cerevisiae.
  44. (1995). Translational control by influenza virus. Identification of cis-acting sequences and trans-acting factors which may regulate selective viral mRNA translation.
  45. (1999). Transport of proteins and RNAs in and out of the nucleus.
  46. (2005). tRNA-linked molecular beacons for imaging mRNAs in the cytoplasm of living cells.
  47. (2004). Viral regulation of mRNA
  48. (2003). Visualizing the distribution and transport of mRNAs in living cells.
  49. (2005). Visualizing the dynamic behavior of poliovirus plus-strand RNA in living host cells.

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.