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Cytotoxicity and reactive oxygen species generation from aggregated carbon and carbonaceous nanoparticulate materials

By Kristine M Garza, Karla F Soto and Lawrence E Murr

Abstract

We have investigated the cytotoxicity and reactive oxygen species (ROS) generation for indoor and outdoor soots: candle, wood, diesel, tire, and natural gas burner soots – along with surrogate black carbon, various multiwall carbon nanotube aggregate materials, TiO2 (anatase) and chrysotile asbestos as reference materials. All soots were observed utilizing TEM and FESEM to be composed of aggregated, primary spherules (20–80 nm diameter) forming complex, branched fractal structures. These spherules were composed of intercalated, turbostratic arrangements of curved graphene fragments with varying concentrations of polycyclic aromatic hydrocarbon (PAH) isomers. In vitro cultures with an immortalized human lung epithelial carcinoma cell line (A549) treated with these materials showed decreased cell viability and variations in ROS production, with no correlations to PAH content. The data demonstrate that soots are cytotoxic and that cytotoxicity is not related to PAH content but is related to ROS generation, suggesting that soot induces cellular oxidative stress and that cell viability assays can be indicators of ROS production

Topics: Original Research
Publisher: Dove Medical Press
OAI identifier: oai:pubmedcentral.nih.gov:2526363
Provided by: PubMed Central

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Citations

  1. (2002). A methodology for measuring size-dependent chemical composition of ultrafi ne particles. Aerosol Sci Technol,
  2. (2006). A review of carbon nanotubes toxicity and assessment of potential occupational and environmental health risks. Crit Rev Toxicol,
  3. (2004). A TEM comparison of chrysotile (asbestos) nanotubes and carbon nanotubes.
  4. and Waste Manage Assoc,
  5. (2005). Atmosphere. Air-pollution-related illness: effects of particles.
  6. (2006). Biological effects of nanoparticulate materials.
  7. (2006). Carbon nanotubes in wood soot. Atmos Sci Lett,
  8. (2004). Carbon nanotubes, nanocrystal forms, and complex nanoparticle aggregates in common fuel gas combustion streams.
  9. (2004). Cardiovascular mortality and long-term exposure to particulate air pollution: epidemiological evidence of general pathophysiological pathways of disease.
  10. (2007). Characterization and comparison of speciated atmospheric carbonaceous (soot) particulates and their polycyclic aromatic hydrocarbon contents in the context of the Paso del Norte airshed along the U.S.-Mexico border. Polycyclic Aromatic Comp,
  11. (2000). Combustion aerosols. Factors governing their size and composition and implications for human health.
  12. (2006). Combustion-generated nanoparticles produced in a benzene fl ame: a multiscale approach.
  13. (2006). Combustion-generated nanoparticulates in the El Paso, TX, USA/Juarez, Mexico metroplex: Their comparative characterization and potential for adverse health effects.
  14. (2005). Comparative in vitro cytotoxicity assessment of some manufactured nanoparticulate materials characterized by transmission electron microscopy.
  15. (2007). Cytotoxic effects of aggregated nanomaterials.
  16. (2008). Cytotoxic responses and potential respiratory health effects of carbon and carbonaceous nanoparticulates in the Paso del Norte airshed environment. Int J Environ Res and Public Health, In press.
  17. (2005). Diabetes enhances vulnerability to particulate air pollution-associated impairment in vascular reactivity and endothelial function.
  18. (1991). Electron and ion nicroscopy and microanalysis: Principles and applications.
  19. (2003). Electron microscopy comparisons of fi ne and ultrafi ne carbonaceous and nano-carbonaceous, airborne particulates. Atmos Environ,
  20. (2004). Fine particles and human health – a review of epidemiological studies. Toxicol Lef,
  21. (1999). Free radicals in biology and medicine.
  22. (1998). Fullerenes and soot formation – New pathways to large particles in fl ames. Angew Chem Int Ed,
  23. (2006). Health effects of fi ne particulate air pollution: Lines that connect.
  24. (2007). Health hazards of manufactured, natural environmental and other anthropogenic atmospheric nanoparticulate materials: past, present and future.
  25. (2002). Infl ammation caused by particles and fi bers. Trans Inhal Toxicol,
  26. (2004). Nanoparticles health effects?
  27. (2006). Nanoparticles: Health effects – pros and cons. Environ Health Perspect,
  28. (2005). Nanotechnology: an energizing discipline evolving from studies of ultrafi ne particles. Environ Health Perspect,
  29. (1998). Outdoor environmental injury of the airways and development of allergic respiratory diseases. Pulmon Pharmacol Ther,
  30. (1995). Particulate air pollution as a predictor of mortality in a perspective study of U.S.
  31. (2005). Pulmonary effects of indoor- and outdoor-generated particles in children with asthma. Environ Health Perspect,
  32. (2001). Pulmonary effects of inhaled ultrafi ne particles. Int Arch Occup Environ Health,
  33. (2004). Pulmonary function, diffusing capacity, and infl ammation in healthy and asthmatic subjects exposed to ultrafi ne particles. Inhal Toxicol,
  34. (2006). Pulmonary instillation studies with TiO2 rods and dots in rats: toxicity is not dependent upon particle size and surface area.
  35. (2004). Relative effects of air pollution on lungs and heart.
  36. (2000). Respir Crit Care Med, 151(part 1):669–74.International
  37. (1997). Short-term effects of particulate air pollution on respiratory morbidity in asthmatic children. Eur RespirJ,
  38. (2004). Soot nanostructure: Dependence upon synthesis conditions. Combust and Flame,
  39. (2006). Speciation of indoor air pollutants and associated inhalation health hazards caused by cooking and heating in Hispanic households, Report to Southwest Consortium for Environmental Research and Policy (SCERP),
  40. (2001). Systemic and cardiovascular effects of airway injury and infl ammation: ultrafi ne particle exposure in humans. Environ Health Perspectives,
  41. (2004). The effect of air pollution on lung development from 10 to 18 years of age.
  42. (2003). The impact of nanoscience on heterogeneous catalysis.
  43. (2002). The surface charge of visible particulate matter predicts biological activation in human bronchial epithelial cells. Toxicol Appl Pharmacol,
  44. (2006). Toxic potential of materials at the nanolevel.
  45. (2006). Traffi c-generated emissions of ultrafi ne particles from pavement – tire interface. Atmos Environ,
  46. (2004). Traffi c-related pollution near busy roads: The East Bay Childrens Respiratory Health Study.
  47. (2004). Ultrafi ne particle deposition in subjects with asthma. Environ Health Perspect,
  48. (2001). Ultrafi ne particles. Occup Environ Med,
  49. (2005). Unusual infl ammatory and fi brogenic pulmonary responses to single-walled carbon nanotubes in mice.
  50. (2003). Utilization of selected area electron diffraction patterns for characterization of air submicron particulate matter collected by a thermal precipitator.
  51. (2001). Volatile organic compounds, polycyclic aromatic hydrocarbons, and elements in the air of ten urban homes. Indoor Air,

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