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Functional analysis via standardized whole-blood stimulation systems defines the boundaries of a healthy immune response to complex stimuli.

By Darragh Duffy, Vincent Rouilly, Valentina Libri, Milena Hasan, Benoit Beitz, Mikael David, Alejandra Urrutia, Aurélie Bisiaux, Samuel T Labrie, Annick Dubois, Ivo G Boneca, Cécile Delval, Stéphanie Thomas, Lars Rogge, Manfred Schmolz, Lluis Quintana-Murci, Matthew L Albert, Laurent Abel, Andres Alcover, Philippe Bousso, Ana Cumano, Marc Daëron, Caroline Demangel, Ludovic Deriano, James Di Santo, Françoise Dromer, Gérard Eberl, Jost Enninga, Antonio Freitas, Ivo Gomperts-Boneca, Serge Hercberg, Olivier Lantz, Claude Leclerc, Hugo Mouquet, Sandra Pellegrini, Stanislas Pol, Anavaj Sakuntabhai, Olivier Schwartz, Benno Schwikowski, Spencer Shorte, Vassili Soumelis, Frédéric Tangy, Eric Tartour, Antoine Toubert and Marie-Noëlle Ungeheuer

Abstract

International audienceStandardization of immunophenotyping procedures has become a high priority. We have developed a suite of whole-blood, syringe-based assay systems that can be used to reproducibly assess induced innate or adaptive immune responses. By eliminating preanalytical errors associated with immune monitoring, we have defined the protein signatures induced by (1) medically relevant bacteria, fungi, and viruses; (2) agonists specific for defined host sensors; (3) clinically employed cytokines; and (4) activators of T cell immunity. Our results provide an initial assessment of healthy donor reference values for induced cytokines and chemokines and we report the failure to release interleukin-1α as a common immunological phenotype. The observed naturally occurring variation of the immune response may help to explain differential susceptibility to disease or response to therapeutic intervention. The implementation of a general solution for assessment of functional immune responses will help support harmonization of clinical studies and data sharing

Topics: MESH : Adaptive Immunity, MESH : Antigens, MESH : Cytokines, MESH : Healthy Volunteers, MESH : Humans, MESH : Immunity, Innate, MESH : Inflammation Mediators, MESH : Monitoring, Immunologic, MESH : Reference Values, MESH : Reproducibility of Results, [ SDV.IMM ] Life Sciences [q-bio]/Immunology
Publisher: Elsevier
Year: 2014
DOI identifier: 10.1016/j.immuni.2014.03.002
OAI identifier: oai:HAL:pasteur-01384537v1
Provided by: Hal-Diderot
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