Klonsko razmnožavanje bundeve (Cucurbita pepo L.) kulturom nodalnih segmenata in vitro

Abstract

Pumpkin (Cucurbita pepo L.) plantlets originated from (6-8 -day-old) seedling shoot tips and somatic embryos — most of them from long-term callus lines, were cloned by nodal explants on agar solidified MS medium without hormones or MS containing IAA and/or BA. The effects of exogenous growt regulators on shoot elongation, root formation and rate of multiplication were investigated. The best development of shoots derived from seedling explants was achieved on MS supplemented with 2.9 pM IAA and 0.22 pM BA, and from somatic embryo shoot explants on MS medium with 5.7 pM IAA. Root formation was obtained on MS without hormones, and MS supplemented with 5.7 pM IAA, or with 2.9 pM IAA and 0.22 pM BA. The last combination of hormones induced callus proliferation at the basal end of shoots but more abundant roots than on medium without BA.Kloniranje biljaka bundeve (Cucurbita pepo L.) u uvjetima in vitro postignuto je na krutoj MS hranidbenoj podlozi bez regulatora rastenja ili uz dodatak IAA i BA u niskim koncentracijama. Za početne eksplantate korišteni su nodalni segmenti biljčica razvijenih iz vegetacijskih vrš- kova 6—8 dana starih klijanaca isklijavanih u uvjetima in vitro ili iz somatskih embrija. Najbolji rast izdanaka i kompletnih biljaka postignut je na podlozi s dodatkom 2,9 PM IAA i 0,22 pM BA, bez obzira na različitu izvornost eksplantata

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