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Molecular Characterization of Porcine Picobirnaviruses and Development of a Specific Reverse Transcription-PCR Assay▿ †

By Gabriela M. Carruyo, Guaniri Mateu, Laura C. Martínez, Flor H. Pujol, Silvia V. Nates, Ferdinando Liprandi and Juan E. Ludert

Abstract

The molecular characterization of partial- length genomic segment 2 of porcine picobirnavirus (PBV) strains and the development of a specific reverse transcription-PCR (RT-PCR) assay for detection of virus in feces are reported. Phylogenetic analysis indicated that the studied porcine isolates were more closely related (>85% identity) to human PBV belonging to genogroup I than to the other porcine PBV described so far. Analysis by RT-PCR and polyacrylamide gel electrophoresis of fecal samples collected in Venezuela and Argentina showed that PBV circulate at high frequencies in piglets

Topics: Virology
Publisher: American Society for Microbiology (ASM)
OAI identifier: oai:pubmedcentral.nih.gov:2446882
Provided by: PubMed Central
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