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Functional Analysis of the Essential GTP-Binding-Protein-Coding Gene cgtA of Vibrio cholerae▿

By Sangita Shah, Bhabatosh Das and Rupak K. Bhadra

Abstract

The cgtA gene, coding for the conserved G protein CgtA, is essential in bacteria. In contrast to a previous report, here we show by using genetic analysis that cgtA is essential in Vibrio cholerae even in a ΔrelA background. Depletion of CgtA affected the growth of V. cholerae and rendered the cells highly sensitive to the replication inhibitor hydroxyurea. Overexpression of V. cholerae CgtA caused distinct elongation of Escherichia coli cells. Deletion analysis indicated that the C-terminal end of CgtA plays a critical role in its proper function

Topics: Genetics and Molecular Biology
Publisher: American Society for Microbiology (ASM)
OAI identifier: oai:pubmedcentral.nih.gov:2446790
Provided by: PubMed Central
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