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The Mycobacterium tuberculosis Rv2540c DNA sequence encodes a bifunctional chorismate synthase

By Fernanda Ely, José ES Nunes, Evelyn K Schroeder, Jeverson Frazzon, Mário S Palma, Diógenes S Santos and Luiz A Basso
Topics: Research Article
Publisher: BioMed Central
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Provided by: PubMed Central

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  1. (1992). A continuous spectrophotometric assay for inorganic phosphate and for measuring phosphate release kinetics in biological systems.
  2. (1998). BG: Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature
  3. (1989). Buckmann AF: Enzymatic synthesis of [4R-2H]NAD(P)H and [4S-2H]NAD(P)H and determination of the stereospecificity of 7 alpha- and 12 alpha-hydroxysteroid dehydrogenase. Biochem Biophys Acta
  4. (2003). Cambillau C: Medium-scale structural genomics: strategies for protein expression and crystallization. Acc Chem Res
  5. (2001). characterization, and overexpression of flavin reductase involved in dibenzothiophene desulfurization by Rhodococcus erythropolis D-1. Appl Environ Microbiol
  6. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature
  7. (2007). Conservation of NADPH utilization by chorismate synthase and its implications for the evolution of the shikimate pathway. Mol Micribiol
  8. (2006). de Azevedo WF Jr: Structure of chorismate synthase from Mycobacterium tuberculosis.
  9. (1983). Double isotope fractionation: test for concertedness and for transition-state dominance. J Am Chem Soc
  10. (2007). DS: Chorismate Synthase: An attractive target for drug development against orphan diseases. Curr Drug Targets
  11. (2001). DS: Cloning and overexpression in soluble form of functional shikimate kinase and 5-enolpyruvylshikimate 3-phosphate synthase enzymes from Mycobacterium tuberculosis. Protein Expr Purif
  12. (2003). DS: One-step purification of 5-enolpyruvylshikimate-3-phosphate synthase enzyme from Mycobacterium tuberculosis. Protein Expr Purif
  13. (2006). for Disease Control and Prevention): Emergence of Mycobacterium tuberculosis with extensive resistance to second-line drugs worldwide. Morb Mortal Wkly Rep
  14. (1990). Guidelines for preventing the transmission of tuberculosis in health care settings, with special focus on HIV-related issues Rep. Morb Mortal Wkly Rep
  15. (1984). High-performance ion-exchange separation of oxidized and reduced nicotinamide adenine dinucleotides. Anal Biochem
  16. (1991). Kinetic and Regulatory Mechanisms of Enzymes from Isotope Effects.
  17. (2005). LA: DAHP synthase from Mycobacterium tuberculosis H37Rv: cloning, expression, and purification of functional enzyme. Protein Expr Purif
  18. (1991). LD: Theoretical Basis and Mechanistic Utility of Solvent Isotope Effects.
  19. (1998). Lobinski R: Characterization of horse kidney metallothionein isoforms by electrospray MS and reversedphase HPLC-electrospray MS. Analyst
  20. (2004). Macheroux P: Mechanism of chorismate synthase.
  21. (2007). Mutagenic analysis of an invariant aspartate residue in chorismate synthase supports its role as an active site base. Biochemistry
  22. NAD(P)H-flavin oxidoreductase from the bioluminescent bacterium, Vibrio fischeri ATCC is a flavoprotein.
  23. (1982). Nutrition, growth and metabolism.
  24. (1990). Observation of an isotope effect in the chorismate synthase reaction.
  25. (2007). ON: A structural model for chorismate synthase from Mycobacterium tuberculosis in complex with coenzyme and substrate. Comput Biol Med
  26. (2006). Organization: Global tuberculosis control: surveillance, planning, financing.
  27. (1998). Osburne MS: Spontaneous cAMP-dependent derepression of gene expression in stationary phase plays a role in recombinant expression instability. Gene
  28. (2005). Pure but not simple. Nature
  29. (1998). Purification and characterization of NfrA1, a Bacillus subtilis nitro/flavin reductase capable of interacting with the bacterial luciferase. Biosci Biotechnol Biochem
  30. (1999). Raviglione MC: Consensus statement. Global burden of tuberculosis: estimated incidence, prevalence, and mortality by country. WHO Global Surveillance and Monitoring Project. JAMA
  31. (1998). Resistance to antitubercular drugs. Adv Exp Med Biol
  32. (1981). RL Schowen: The use of isotope effects to elucidate enzyme mechanisms. BioScience
  33. (2007). Rodrigues-Pousada C: Molecular determinants for FMN-binding in Desulfovibrio gigas flavoredoxin. FEBS Letters
  34. (1996). RP: A spectrophotometric method to measure enzymatic activity in reactions that generate inorganic pyrophosphate. Anal Biochem
  35. (1996). Saccharomyces cerevisiae chorismate synthase has a flavin reductase activity. Mol Microbiol
  36. (2004). SH: Kinetic and chemical mechanisms of the fabG-encoded Streptococcus pneumoniae β-ketoacyl-ACP reductase. Biochemistry
  37. (2001). Spectroscopic and kinetic characterization of the bifunctional chorismate synthase from Neurospora crassa.
  38. (1975). Steady-state analysis of kinetic isotope effects in enzymic reactions. Biochemistry
  39. (2004). SW: Crystal structure of chorismate synthase: A novel FMN-binding protein fold and functional insights.
  40. (2002). The common aromatic amino acid biosynthesis pathway is essential in Mycobacterium tuberculosis.
  41. (2002). The future challenges facing the development of new antimicrobial drugs. Nat Rev Drug Discov
  42. (2003). The growing burden of tuberculosis. Arch Intern Med
  43. (1988). The overexpression, purification and complete amino acid sequence of chorismate synthase from Escherichia coli K12 and its comparison with the enzyme from Neurospora crassa.
  44. (1990). The shikimate pathway – a metabolic tree with many branches. Crit Rev Biochem Mol Biol
  45. (1995). The shikimate pathway: early steps in the biosynthesis of aromatic compounds. Plant Cell
  46. (2003). The structure of chorismate synthase reveals a novel flavin binding site fundamental to a unique chemical reaction. Structure
  47. (1996). Thorneley RNF: Binding of the oxidized, reduced, and radical flavin species to chorismate synthase. An investigation by spectrophotometry, fluorimetry, and electron paramagnetic resonance and electron nuclear double resonance spectroscopy. Biochemistry
  48. (1990). Thorneley RNF: Chorismate synthase: pre-steady-state kinetics of phosphate release from 5-enolpyruvylshikimate 3-phosphate. Biochem J
  49. (2002). TR: Controlling multidrugresistant tuberculosis and access to expensive drugs: a rational framework. Bulletin of the World Health Organization
  50. (1976). WL: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem
  51. (1984). WW: Use of multiple isotope effects to determine enzyme mechanisms and intrinsic isotope effects. Biochemistry

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