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Curcuma longa extract as a histological dye for collagen fibres and red blood cells

By O G Avwioro, S K Onwuka, J O Moody, J M Agbedahunsi, T Oduola, O E Ekpo and A A Oladele


Crude ethanolic extract and column chromatographic fractions of the Allepey cultivar of Curcuma longa Roxb, commonly called turmeric (tumeric) in commerce, were used as a stain for tissue sections. Staining was carried out under basic, acidic and neutral media conditions. Inorganic and organic dissolution solvents were used. The stain was used as a counterstain after alum and iron haematoxylins. C. longa stained collagen fibres, cytoplasm, red blood cells and muscle cells yellow. It also stained in a fashion similar to eosin, except for its intense yellow colour. Preliminary phytochemical evaluation of the active column fraction revealed that it contained flavonoids, free anthraquinone and deoxy sugar. A cheap, natural dye can thus be obtained from C. longa

Topics: Methods
Publisher: Blackwell Publishing Inc
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Provided by: PubMed Central
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