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Down-regulation of TGF- β 1 production restores immunogenicity in prostate cancer cells

By E Matthews, T Yang, L Janulis, S Goodwin, S D Kundu, W J Karpus and C Lee

Abstract

The objective of this study is to determine if a non-immunogenic Dunning’s rat prostate cancer cell line, MATLyLu, can become immunogenic by reducing the endogenous production of TGF-β1. An expression construct containing a DNA sequence in an antisense orientation to TGF-β1 (TGF-β1 antisense) was stably transfected into MATLyLu cells. Following transfection, cellular content of TGF-β1 reduced from 70 to10 pg per 2 × 104cells and the rate of in vitro3H-thymidine incorporation increased 3–5-fold. After subcutaneous injection of tumour cells into syngeneic male hosts (Copenhagen rats), the tumour incidence was 100% (15/15) for the wild type MATLyLu cells and cells transfected with the control construct, but only 43% (9/21, P≤ 0.05) for cells transfected with TGF-β1 antisense. However, when cells were injected into immunodeficient hosts (athymic nude rats), the incidence of tumour development was 100% (10/10) for both the wild type MATLyLu cells and cells transfected with the control construct and 90% (9/10) for cells transfected with TGF-β1 antisense. These observations support the concept that MATLyLu cells are immunogenic, when the endogenous production of TGF-β1 is down-regulated. © 2000 Cancer Research Campaig

Topics: Regular Article
Publisher: Nature Publishing Group
OAI identifier: oai:pubmedcentral.nih.gov:2374659
Provided by: PubMed Central

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Citations

  1. (1977). Characterization of the Dunning R3327H prostatic adenocarcinoma: An appropriate animal model for prostate cancer. Cancer Treat Rep 61: 273–287
  2. (1997). TGF-b in prostate cancer: A growth inhibitor that can enhance tumourigenicity.
  3. (1990). Transforming growth factor-b1-induced changes on cell migration, proliferation, and angiogenesis in the chicken chorioallantoic membrane.