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Possible Involvement of Endothelin Peptides and L-Arginine-Nitric Oxide Pathway on the Effect of Endotoxin in the Rabbit Isolated Perfused Kidney

By K. Özsan, R. K. Türker and Z. S. Ercan


Escherichia coli endotoxin (LPS) when infused through the renal artery of the rabbit isolated perfused kidney prepared as constant pressure mode, caused a decrease in flow rate and kidney weight indicating its primary vasoconstrictor effect. This effect was predominant in kidneys from rabbits pretreated with LPS. Endothelin-1 at a concentration of 10−10 M and big endothelin-1 at a concentration of 10−8 M produced equal vasoconstrictor effects in kidney. Addition of endotheHn converting enzyme inhibitor, phosphoramidon, to the perfusion medium at a concentration of 10−6 M caused a reduction in the effects of both LPS and big ET-1 without altering the vasoconstrictor effect of ETol. However, addition of methylene blue (10−5 M), a soluble guanylate cyclase inhibitor and NG-nitro-L-arginine-methyl ester (10−6 M) to the perfusion medium caused a potentiation in the vasoconstrictor effect of LPS. Indomethacin at a concentration of 10−6 M did not alter the effect of LPS. These results were taken as evidence for the participation of endothelin peptides and the L-arginine-nitric oxide pathway in the effect ofLPS in rabbit isolated perfused kidney

Topics: Research Article
Publisher: Hindawi Publishing Corporation
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Provided by: PubMed Central

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  1. (1987). Alterations in vascular reactivity in sepsis and endotoxemia. In:
  2. (1990). Antiinflammatory glucocorticoids inhibit the induction by endotoxin of nitric oxide synthase in the lung, liver and aorta of the rat.
  3. (1990). Conversion of big endothelin-1 to endothelin-1 by membrane bound metalloproteinase in cultured bovine endothelial cells. Biochem Biophys Res Commun
  4. (1991). Conversion of big endothelin-1 to endothelin-1 by two types of metalloproteinases of cultured porcine vascular smooth muscle cells. Biochem Biophys Res Commun
  5. Cultured endothelial cells maintain their L-arginine level despite the continuous release of EDRF.
  6. (1988). Demirel E, Ercan ZS. Iloprost preserves kidney functions against anoxia. Proc Leuk Essen Fatty Acids
  7. (1986). Direct effects of E. coli endotoxin structure and permeability of pulmonary endothelial monolayers and the endothelial layer of initial explant.
  8. (1986). Endotoxemic acute renal failure in awake rats.
  9. (1989). Endotoxin stimulates endothelin-release in vivo and in vitro determined by radioimmunoassay.
  10. (1990). Glucocorticoids inhibit the expression of inducible, but not the constitutive, nitric oxide synthase in vascular endothelial cells.
  11. (1989). Increased plasma and pulmonary lymph levels of endothelin during endotoxin shock.
  12. (1990). Inhibition of nitric oxide synthesis reduces the hypotension induced by bacterial lipopolysaccharides in the rat in vivo.
  13. (1992). L-Arginine induces relaxation of small mesenteric arteries from endotoxin-treated rats. EurJPharmacol
  14. (1990). metalloproteinase inhibitor, suppresses the secretion of endothelin-1 from cultured endothelial cells by inhibiting big endothelin-1 converting enzyme. Biochem Biophys Res Commun
  15. (1992). Modifications of -adrenoceptor-mediated pressor responses by Na-nitro-l:arginine methyl ester and vasopressin in endotoxin-treated pithed rats. EurJPharmacol
  16. (1991). Nitric oxide: physiology, pathophysiology and pharmacology. Pharmacol Rev
  17. (1990). Possible involvement of endothelium in the responses of various vasoactive agents in rabbit isolated perfused kidney. Gen Pharmacol
  18. (1994). Role of endothelium in haemoglobin-induced contraction of dog basilar artery. EurJPharmacol 1987; 140: 105-108. ACKNOWLEDGEMENT. This work supported by grant from
  19. (1985). Selective blockade of endothelium-dependent and glyceryl trinitrate induced relaxation by hemoglobin and methylene blue in rabbit aorta. J Pharmacol Exp Ther
  20. (1991). The effect of inhibitors of the L-arginine/nitric oxide pathway endotoxin-induced loss of vascular responsiveness in anaesthetized rats. BrJPharmacol