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Cloning, expression, crystallization and preliminary X-ray analysis of the first two Ig domains from human Roundabout 1 (Robo1)

By Cecile Morlot, Wieger Hemrika, Roland A. Romijn, Piet Gros, Stephen Cusack and Andrew A. McCarthy

Abstract

Robo1 is a large transmembrane receptor expressed on the axon growth cone. Activation of Robo1 by Slit proteins results in axon repulsion from the midline. Here, the purification and crystallization conditions of first two Ig domains of Robo1 are reported

Topics: Crystallization Communications
Publisher: International Union of Crystallography
OAI identifier: oai:pubmedcentral.nih.gov:2335160
Provided by: PubMed Central
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