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Identification of genes potentially involved in supporting hematopoietic stem cell activity of stromal cell line MC3T3-G2/PA6

By Natsumi Shimizu, Shinichi Noda, Kazufumi Katayama, Hitoshi Ichikawa, Hiroaki Kodama and Hiroyuki Miyoshi


Although coculture of hematopoietic stem cells (HSCs) with stromal cells is a useful system to study hematopoiesis in the niche, little is known regarding the precise cellular and molecular mechanisms of maintaining HSCs through cell–cell interactions. The murine preadipose stromal cell line MC3T3-G2/PA6 (PA6) has been demonstrated to support HSCs in vitro. In this study, microarray analysis was performed on PA6 cells and HSC-nonsupporting PA6 subclone cells to identify genes responsible for supporting HSC activity. Comparison of gene expression profiles revealed that only 144 genes were down-regulated by more than twofold in PA6 subclone cells. Of these down-regulated genes, we selected 11 candidate genes and evaluated for the maintenance of HSC function by overexpressing these genes in PA6 subclone cells. One unknown gene, 1110007F12Rik (also named as Tmem140), which is predicted to encode an integral membrane protein, demonstrated a partial restoration of the defect in HSC-supporting activity

Topics: Original Article
Publisher: Springer Japan
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Provided by: PubMed Central

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  1. (1993). A murine stromal cell line allows the proliferation of very primitive human CD34++/CD38- progenitor cells in long-term cultures and semisolid assays.
  2. A new preadipose cell line derived from newborn mouse calvaria can promote the proliferation of pluripotent hemopoietic stem cells in vitro.
  3. A stromal cell line from myeloid longterm bone marrow cultures can support myelopoiesis and B lymphopoiesis.
  4. (2004). Clinical strategies for expansion of haematopoietic stem cells. Nat Rev Immunol.
  5. (1991). Differential regulation of primitive human hematopoietic cells in long-term cultures maintained on genetically engineered murine stromal cells.
  6. (1996). Ex vivo expansion of murine hematopoietic progenitor cells generates classes of expanded cells possessing different levels of bone marrow repopulating potential. Exp Hematol.
  7. Gene expression in stem cell-supporting stromal cell lines.
  8. (1997). Gp130 and the interleukin-6 family of cytokines. Annu Rev Immunol.
  9. (1998). Hematopoietic stem cell maintenance and differentiation are supported by embryonic aorta-gonad-mesonephros region-derived endothelium.
  10. (2000). Human hematopoietic stem cells stimulated to proliferate in vitro lose engraftment potential during their S/G(2)/M transit and do not reenter G(0).
  11. In vitro and in vivo expansion of hematopoietic stem cells.
  12. (1992). In vitro proliferation of primitive hemopoietic stem cells supported by stromal cells: evidence for the presence of a mechanism(s) other than that involving c-kit receptor and its ligand.
  13. (1994). Involvement of the c-kit receptor in the adhesion of hematopoietic stem cells to stromal cells. Exp Hematol.
  14. (1992). Isolation of a candidate human hematopoietic stem-cell population.
  15. (1997). Lemischka IR. In vitro maintenance of highly purified, transplantable hematopoietic stem cells.
  16. (2002). Lentiviral vector-mediated transduction of murine CD34(-) hematopoietic stem cells. Exp Hematol.
  17. (1996). Long-term lymphohematopoietic reconstitution by a single CD34-low/negative hematopoietic stem cell.
  18. (1994). Long-term reconstitution of mice after ex vivo expansion of bone marrow cells: differential activity of cultured bone marrow and enriched stem cell populations. Exp Hematol.
  19. (1995). Murine marrow cells expanded in culture with IL-3, IL-6, IL-11, and SCF acquire an engraftment defect in normal hosts. Exp Hematol.
  20. (1997). Quantitative analysis reveals expansion of human hematopoietic repopulating cells after short-term ex vivo culture. J Exp Med.
  21. (1998). Stimulation of mouse and human primitive hematopoiesis by murine embryonic aorta-gonadmesonephros-derived stromal cell lines.