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Two yellow-pigmented, Gram-negative, rod-shaped bacterial strains, GH1-10T and GH29-5T, were isolated from greenhouse soils in Korea. 16S rRNA gene sequence analysis indicated that these strains were related to members of the genus Flavobacterium. Strain GH1-10T was most closely related toFlavobacterium psychrolimnae and Flavobacteriumdenitrificans, with sequence similarities of 95?9 and 95?2%, respectively. Strain GH29-5T was most closely related to ‘Flavobacterium saliodium’, F. denitrificans and Flavobacterium frigoris, with sequence similarities of 94?3, 92?5 and 92?5%, respectively. The major cellular fatty acids of GH1-10T were iso-C15:0, summed feature 3 (iso-C15: 0 2-OH and/or C16: 1v7c) and iso-C17: 0 3-OH, and those of GH29-5 T were iso-C15: 0, iso-C17: 0 3-OH, iso-C15: 1 G and iso-C15:0 3-OH. Both strains contained menaquinone with six isoprene units (MK-6) as the sole quinone. TheDNAG+Ccontents of GH1-10T andGH29-5Twere 35 and 39 mol%, respectively. Based on the phylogenetic and phenotypic data presented, it is concluded that the two bacteria represent two separate novel species of the genus Flavobacterium. The names proposed to accommodate these organisms are Flavobacterium daejeonense sp. nov., with type strainGH1-10T (=KACC11422T=DSM17708T), andFlavobacterium suncheonense sp

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