Letter to the Editor Effect of extracellular HIV-1 Vpr protein in vitro


We have noted the article by Huang et al (2000) with great interest and are pleased to see our previous nd-ings conrmed in another laboratory. We read with interest that extracellular Vpr does not cause G2/M arrest at least in C6 glioma cells, conrming our data that it localizes at the cell membrane. However, there are several points that are unclear. The statement that neither Annexin V nor TUNEL staining are enough to discriminate between necro-sis and apoptosis is perplexing as these are the most widely used assays in the apoptosis eld. Seeing that Annexin V, TUNEL, and DNA fragmentation pointed towards apoptosis, it is not apparent why Huang et al claim the effects of Vpr to be more necrotic than apoptotic in the abstract

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