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Sequence and organization of the Spodoptera exigua multicapsid nucleopolyhedrovirus genome

By Wilfred F. J. Ijkel, Elisabeth A. Van Strien, Jacobus G. M. Heldens, Rene Broer, Douwe Zuidema, Rob W. Goldbach and Just M. Vlak


The nucleotide sequence of the DNA genome of Spodoptera exigua multicapsid nucleopoly-hedrovirus (SeMNPV), a group II NPV, was determined and analysed. The genome contains 135611 bp and has a GMC content of 44 mol%. Computer-assisted analysis revealed 139 ORFs of 150 nucleotides or larger; 103 have homologues in Autographa californica MNPV (AcMNPV) and a further 16 have homologues in other baculoviruses. Twenty ORFs are unique to SeMNPV. Major differences in SeMNPV gene content and arrangement were found compared with the group I NPVs AcMNPV, Bombyx mori (Bm) NPV and Orgyia pseudotsugata (Op) MNPV and the group II NPV Lymantria dispar (Ld) MNPV. Eighty-five ORFs were conserved among all five baculoviruses and are considered as candidate core baculovirus genes. Two putative p26 and odv-e66 homologues were identified in SeMNPV, each of which appeared to have been acquired independently and not by gene duplication. The SeMNPV genome lacks homologues of the major budded virus glycoprotein gene gp64, the immediate-early transactivator ie-2 and bro (baculovirus repeat ORF) genes that are found in AcMNPV, BmNPV, OpMNPV and LdMNPV. Gene parity analysis of baculovirus genomes suggests that SeMNPV and LdMNPV have a recent common ancestor and that they are more distantly related to the group I baculoviruses AcMNPV, BmNPV and OpMNPV. The orientatio

Year: 1999
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