Article thumbnail

Molecular Proximity of Kv1.3 Voltage-gated Potassium Channels and β1-Integrins on the Plasma Membrane of Melanoma Cells: Effects of Cell Adherence and Channel Blockers

By Vira V. Artym and Howard R. Petty

Abstract

Tumor cell membranes have multiple components that participate in the process of metastasis. The present study investigates the physical association of β1-integrins and Kv1.3 voltage-gated potassium channels in melanoma cell membranes using resonance energy transfer (RET) techniques. RET between donor-labeled anti–β1-integrin and acceptor-labeled anti-Kv1.3 channels was detected on LOX cells adherent to glass and fibronectin-coated coverslips. However, RET was not observed on LOX cells in suspension, indicating that molecular proximity of these membrane molecules is adherence-related. Several K+ channel blockers, including tetraethylammonium, 4-aminopyridine, and verapamil, inhibited RET between β1-integrins and Kv1.3 channels. However, the irrelevant K+ channel blocker apamin had no effect on RET between β1-integrins and Kv1.3 channels. Based on these findings, we speculate that the lateral association of Kv1.3 channels with β1-integrins contributes to the regulation of integrin function and that channel blockers might affect tumor cell behavior by influencing the assembly of supramolecular structures containing integrins

Topics: Article
Publisher: The Rockefeller University Press
OAI identifier: oai:pubmedcentral.nih.gov:2311400
Provided by: PubMed Central

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.

Suggested articles

Citations

  1. (2002). Activation-induced conformational changes in the I domain region of lymphocyte function-associated antigen 1.
  2. (1999). Activity of voltage-gated K channels is associated with cell proliferation and Ca2 influx in carcinoma cells of colon cancer. Life Sci.
  3. (1994). An engineered cysteine in the external mouth of a K channel allows inactivation to be modulated by metal binding.
  4. (1993). Biology of disease. Role of integrins and other cell adhesion molecules in tumor progression and metastasis.
  5. (2000). Block of the lymphocyte K channel mKv1.3 by the phenylalkylamine verapamil: kinetic aspects of block and disruption of accumulation of block by a single point mutation.
  6. (1997). Combination chemotherapy with cyclophospamide, vincristine, adriamycin, and dexamethason (CVAD) plus oral quinine and verapamil in patients with advanced breast cancer. Breast Cancer Res.
  7. (2001). Dissipative metabolic patterns respond during neutrophil transmembrane signaling.
  8. (2000). Dissipative metabolic structures in living cells: observation of target patterns during cell adherence.
  9. (2000). Effects of voltage-gated ion channel modulators on rat prostatic cancer cell proliferation: comparison of strongly and weakly metastatic cell line.
  10. (1998). Evidence for a functional interaction between integrins and G protein-activated inward rectifier K channels.
  11. (1996). Evidence for an internal phenylalkylamine action on the voltage-gated potassium channel Kv1.3.
  12. (2000). Extracellular K and opening of voltage-gated potassium channels activate T cell integrin function: physical and functional association between Kv1.3 channels and 1 integrins.
  13. (1991). Fibronectin-integrin binding promotes hyperpolarization of murine erythroleukemia cells.
  14. (1987). Fluorescence energy transfer and membrane potential measurements monitor dynamic properties of cell membranes: A critical review.
  15. (2001). HERG K channels activation during 1 integrin-mediated adhesion to fibronectin induces an upregulation of v 3 integrin in the preosteclastic leukemia cell line FLG 29.1.
  16. (2000). Imaging sustained dissipative patterns in the metabolism of individual living cells.
  17. (1996). Induction of human myeloblastic ML-1 cell G1 arrest by suppression of K channel activity.
  18. (1991). Inhibition of tumor cell invasion by verapamil. Pigment Cell Res.
  19. (1993). Inside-out integrin signaling in macrophages.
  20. (1993). Integrin-mediated neurite outgrowth in neuroblastoma cells depends on the activation of potassium channels.
  21. (1990). Integrins and tumor invasion.
  22. (1992). Integrins: versatility, modulation, and signaling in cell adhesion.
  23. (2002). Interactions of integrins with their partner proteins in leukocyte membranes. Immunologic Res.
  24. (1996). K channels and the intracellular calcium signal in human melanoma cell proliferation.
  25. (1999). K( ) channel-dependent migration of fibroblasts and human melanoma cells.
  26. (1990). Mechanisms of potassium channel block in rat alveolar epithelial cells.
  27. (1993). Mechanotransduction across the cell surface and through the cytoskeleton.
  28. (1999). Modulation of glioma cell migration and invasion using Cl and K ion channel blockers.
  29. (1989). Mutual contact of murine erythroleukemia cells activates depolarizing cation channels, whereas contact with extracellular substrata activates hyperpolarizing Ca2 -dependent K channels.
  30. (2001). Neutrophil oscillations: temporal and spatiotemporal aspects of cell behavior. Immunologic Res.
  31. Oscillating activity of Ca2 -sensitive K channel.
  32. (1994). Pharmacalogical characterization of five cloned voltage-37 Artym and Petty gated K channels, types Kv1.1, 1.2, 1.3, 1.5, and 3.1, stably expressed in mammalian cell lines.
  33. (1993). Physiological role, molecular structure and evolutionary relationships of voltage-gated potassium channels in T-lymphocytes.
  34. (1997). Plasminogen activators, integrins, and the coordinated regulation of cell adhesion and migration.
  35. (1995). Potassium and calcium channels in lymphocytes.
  36. (1992). Response to fibronectin-integrin interaction in leukaemia cells: delayed enhancing of a K
  37. (2000). Sensitivity of native and cloned hippocampal delayed-rectifier potassium channels to verapamil.
  38. (1996). Soluble or bound laminin elicit in human neuroblastoma cells short- or long-term potentiation of a K inwardly rectifying current: relevance to neuritogenesis. Cell Adhes.
  39. (2001). Suppression by verapamil of bombensinenhanced peritoneal metastasis of intestinal adenocarcinomas induced by azoxymethane in Wistar rats.
  40. (1999). The effect of deep pore mutation on the action of phenylalkylamines on the Kv1.3 potassium channel.
  41. (1990). The luminal K channel of the thick ascending limb of Henle’s loop. Pflugers Arch.
  42. (1997). Urokinase-type plasminogen activator receptors associate with 1 and 3 integrins of fibrosarcoma cells: dependence on extracellular matrix components. Cancer Res.
  43. (2001). Verapamil inhibits proliferation of LNCaP human prostate cancer cells influencing K channel gating.
  44. (1998). Verapamil inhibits tumor protease production, local invasion and metastasis development in murine carcinoma cells.
  45. (1991). Verapamil-induced blockade of voltage-activated K current in smallcell lung cancer cells.
  46. (1985). Voltage-dependent ion channels in T-lymphocytes.
  47. (1994). Voltageand time-dependent K channel currents in the basolateral membrane of villus enterocytes isolated from guinea pig small intestine.