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Improved determination of plasmid copy number using quantitative real-time PCR for monitoring fermentation processes

By Mihaela Škulj, Veronika Okršlar, Špela Jalen, Simona Jevševar, Petra Slanc, Borut Štrukelj and Viktor Menart
Topics: Research
Publisher: BioMed Central
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Provided by: PubMed Central

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  1. (2001). A new mathematical model for relative quantification in real-time RT-PCR.
  2. (2006). Absolute and relative QPCR quantification of plasmid copy number in Escherichia coli.
  3. (2000). Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays.
  4. (2002). Accotto GP: Estimating the number of integrations in transformed plants by quantitative real-time PCR.
  5. (1998). Amplification of ColE1 related plasmids in recombinant cultures of Escherichia coli after IPTG induction.
  6. (2003). Berdal KG: PCR technology for screening and quantification of genetically modified organisms (GMOs). Anal Bioanal Chem
  7. (2006). Current and emerging molecular diagnostic technologies applicable to bacterial food safety.
  8. (2005). Deb JK: Purification of streptomycin adenylyltransferase from a recombinant Escherichia coli. Protein Expression and Purification
  9. (2004). Detection and traceability of genetically modified organisms in the food production chain. Food Chem Toxicol
  10. (1989). Determination of Protein Expression and Plasmid Copy Number from Cloned Genes in Escherichia-Coli by Flow-Injection Analysis Using An Enzyme Indicator Vector. Biotechnology and Bioengineering
  11. (2005). Directed evolution of copy number of a broad host range plasmid for metabolic engineering. Metabolic Engineering
  12. (1998). Effect of lysing methods and their variables on the yield of Escherichia coli O157: H7 DNA and its PCR amplification.
  13. (1994). Falkow S: Plasmids. In Methods for general and molecular bacteriology Edited by: Gerhardt
  14. (1996). Flaschel E: Rapid determination of plasmid copy number.
  15. (2004). Folch JM: Real-time quantitative PCR-based system for determining transgene copy number in transgenic animals. Biotechniques
  16. (2005). Gajovic S: An improved method for determination of gene copy numbers in transgenic mice by serial dilution curves obtained by real-time quantitative PCR assay.
  17. (2002). Impact of targeted vector design on ColE1 plasmid replication. Trends in Biotechnology
  18. (1987). Isolation of high-molecularweight nucleic acids for copy number analysis using high-performance liquid chromatography.
  19. (2004). IT: Use of real-time PCR for determining copy number and zygosity in transgenic plants. Plant Cell Rep
  20. (2007). Mergulhao FJM: Time-course determination of plasmid content in eukaryotic and prokaryotic cells using Real-Time PCR. Molecular Biotechnology
  21. (2006). ML: The copy-number of plasmids and other genetic elements can be determined by SYBR-Green-based quantitative real-time PCR.
  22. (1983). Novick RP: Determination of plasmid copy number by fluorescence densitometry. Plasmid
  23. (2002). Optimizing recombinant microbial fermentation processes – An integrated approach. Biopharm - The Applied Technologies of Biopharmaceutical Development
  24. (2004). Plasmid copy number and plasmid stability. Adv Biochem Eng Biotechnol
  25. (1979). Plasmid copy number control: isolation and characterization of high-copynumber mutants of plasmid pE194.
  26. (2002). Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems.
  27. (2001). Quantitative real-time PCR assay for determining transgene copy number in transformed plants. Biotechniques
  28. (2005). Rapid detection of diarrheagenic E. coli by real-time PCR.
  29. (1993). Ruusala T: The silent P mating type locus in fission yeast contains two autonomously replicating sequences. Nucleic Acids Res
  30. (2004). Schierholz JM: A quantitative realtime PCR assay for the detection of tetR of
  31. (2001). Shafique W: Comparison of culture, PCR and immunoassays for detecting Escherichia coli O157 following enrichment culture and immunomagnetic separation performed on naturally contaminated raw meat products.
  32. (2006). SK: Quantitative real-time polymerase chain reaction for determination of plasmid copy number in bacteria.
  33. (2006). T: Detection and monitoring of virus infections by real-time PCR. Mol Aspects Med
  34. (2004). The replication-related organization of bacterial genomes.
  35. (2005). Zumla A: Real-time RT-PCR normalisation; strategies and considerations. Genes Immun