The major RNA in prosomes * of HeLa cells and duck erythroblasts is tRNALys3

Abstract

Two-dimensional gel electrophoresis of HeLa cell prosomal RNAs, 3'-end labeled by RNA llgase, revealed one prominent spot. Determination of a partial sequence at the 3'-end Indicated full homology to the 18 nucleotides at the 3-end of tRNA1-**-3 from rabbit, the bovine and the human species. An oligonucleotide complementary to the 3'-end of tRNAL*»-3 hybridized on Northern blots with prosomal RNA from both HeLa cells and duck erythroblasts. In two-dimensional PA-GE, the major pRNA of HeLa cells co-migrated with bovine tRNA1-"-3. Reconstitution of the CCA 3'-end of RNA from both human and duck prosomes, by tRNA-nucleotidyl-transferase, confirmed the tRNA character of this type of RNA. Furthermore, it revealed at least one additional tRNA band about 85 nt long among the prosomal RNA from both species. Finally, confirming an original property of prosomal RNA, we show that in vitro synthesized tRNA"-*9-3 hybridizes stably to duck globin mRNA, and to poly(A)+- and poly(A)~-RNA from HeLa cells

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