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Effect of diluent and storage time on pregnancy rate in ewes after intra-uterine insemination

By J. E. Oliver, J. Parr, F. A. Veldhuizen and G. C. Upreti


Four trials involving the intra-uterine insemination of a total of 840 Coopworth ewes were conducted to assess the fertilization capacity of ram semen stored at WC. Semen from Polled Dorset rams was collected by artificial vagina, diluted with either a standard milk diluent or a synthetic diluent (RSD- 1) to a concentration of 200x 106 sperm/ml, cooled to 15“C, placed in 0.25 ml straws and held at WC for various periods [Day 0 (4h), Day 1 (28 h), Day 2 (52 h), Day 3 (76 h) and Day 4 ( 100 h)] before insemination. Ewes were synchronised, with CIDR devices inserted for 14days and 400 i.u. PMSG was given at time of device removal. Laparoscopic assisted inn-a-uterine inseminations were performed between 52 h and 56 h after device withdrawal. A total of six inseminators were used throughout the trial series with between 3 to 5 inseminators per trial. Conception rates were determined by plasma progesterone levels at day 19 and pregnancy confirmed by real time ultrasonic scanning at day 50 post mating. Thereweresigni~cantdifferencesbetweentrialsintheproportionofewestreatedthatexhibitedoestruspriortoinseminationwithlower percentages in the non-breeding season. There was considerable discrepancy between the two methods of pregnancy detection. A high proportion of the non-tupped ewes deemed pregnant by progesterone were not pregnant at sqtnning. There were no differences between trials in the proportion of ewes pregnant after insemination with Day 0 semen. There were no significant differences between the diluents at any particular time of storage however, the overall pregnancy rate was higher for the milk diluent (SO. 1 % v 45.0%). There was a significant effect of storage time, with the mean overall values for storage periods of 0, 1,2,3 and 4 days being 49.4,46.5,40.3,29.6 and 20.0 % respectively

Year: 2016
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