Article thumbnail

Vaccinia virus infection induces a stress response that leads to association of Hsp70 with viral proteins

By Satish Jindal, Richard and A. Young


We studied the impact of vaccinia virus infection on stress protein gene expression in human cells and investigated the possibility that eukaryotic heat shock proteins interact with viral components during assembly. Infection of human monocyte-macrophages by vaccinia virus caused a dramatic decrease in levels of cellular mRNAs such as those encoding actin and tubulin. In contrast, infection did not cause a significant reduction in the levels of Hsp9O and Hsp6O mRNAs and led to substantially increased levels of Hsp7O mRNAs. The accumulation of these stress protein mRNAs was due both to increases in their transcription rate and to their stability relative to other cellular mRNAs. The relative levels of the heat shock proteins and the other cellular proteins reflected the relative levels of their mRNAs. These results indicate that stress protein gene expression is relatively refractory to the generally deleterious effects ofvaccinia virus infection on host cell gene expression. The continued expression of some of these stress proteins may be beneficial to the virus; the observations that the levels of Hsp7O are greatest at the peak of viral gene expression and that a large fraction of cellular Hsp7O is associated with vaccinia virus proteins suggest that Hsp7O is involved in vaccinia virus assembly. Prokaryotic and eukaryotic cells respond to potentially damaging stimuli by increasing the synthesis of a family of proteins collectively known as stress proteins. The bes

Year: 1992
OAI identifier: oai:CiteSeerX.psu:
Provided by: CiteSeerX
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • (external link)
  • (external link)
  • (external link)
  • Suggested articles

    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.