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P92-M The ABI 3730 DNA Analyzer is an Efficient System for Simultaneous, Bi-Directional Analysis of Dual-Labeled Probes Used to Study Protein-DNA Interactions

By G. L. Spangler


DNA footprinting is a powerful tool to study regions of DNA where proteins bind. Traditionally, target DNA fragments are labeled with 32P, incubated with the protein of interest, then subjected to chemical nuclease attack. The DNA probe is then purified and nicked fragments analyzed on sequencing gels to reveal regions of DNA protected by the protein from attack

Topics: Poster Abstracts: Macromolecular Interactions
Publisher: The Association of Biomolecular Resource Facilities
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Provided by: PubMed Central
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