Article thumbnail

P92-M The ABI 3730 DNA Analyzer is an Efficient System for Simultaneous, Bi-Directional Analysis of Dual-Labeled Probes Used to Study Protein-DNA Interactions

By G. L. Spangler

Abstract

DNA footprinting is a powerful tool to study regions of DNA where proteins bind. Traditionally, target DNA fragments are labeled with 32P, incubated with the protein of interest, then subjected to chemical nuclease attack. The DNA probe is then purified and nicked fragments analyzed on sequencing gels to reveal regions of DNA protected by the protein from attack

Topics: Poster Abstracts: Macromolecular Interactions
Publisher: The Association of Biomolecular Resource Facilities
OAI identifier: oai:pubmedcentral.nih.gov:2292066
Provided by: PubMed Central
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://www.pubmedcentral.nih.g... (external link)

  • To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.

    Suggested articles