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P91-S Investigating Assembly of HIV—Gag Protein with Oligonucleotides using a Quartz Crystal Microbalance with Dissipation Monitoring

By J. Simpson, A. Jaiswal, A. Stephen, S. Datta, A. Rein and R. Fisher


The structural protein group-specific antigen (Gag) is the only viral product required for retrovirus assembly and will form virus-like particles (VLPs) in vitro in the presence of nucleic acids. The nature of the contribution of nucleic acid to particle assembly and structure is not fully understood. Further, interactions of Gag protein with nucleic acids have not been characterized in any detail. We have utilized a quartz crystal microbalance with dissipation monitoring (QCM-D) to investigate the possible assembly of Gag particles in the presence of three oligonucleotides—TGx2.5, TGx10, and TGx20 (a 5-mer, 20-mer, and 40-mer, respectively). QCM-D provides information on both mass changes, measured as a change in frequency, and structural changes measured as changes in energy dissipation

Topics: Poster Abstracts: Macromolecular Interactions
Publisher: The Association of Biomolecular Resource Facilities
OAI identifier: oai:pubmedcentral.nih.gov:2291808
Provided by: PubMed Central
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