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Olfactory receptors are displayed on dog mature sperm cells

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Abstract

Olfactory receptors constitute a huge family of structurally related G protein-coupled receptors, with up to a thousand members expected. We have shown previously that genes belonging to this family were expressed in the male germ line from both dog and human. The functional significance of this unexpected site of expression was further investigated in the present study. We demonstrate that a few dog genes representative of various subfamilies of olfactory receptors are expressed essentially in testis, with little or no expression in olfactory mucosa. Other randomly selected members of the family show the expected site of expression, restricted to the olfactory system. Antibodies were generated against the deduced amino acid sequence of the most abundantly expressed olfactory receptor gene in dog testis. The purified serum was able to detect the gene product (DTMT receptor) in late round and elongated spermatids, as well as in the cytoplasmic droplet that characterizes the maturation of dog sperm cells, and on the tail midpiece of mature spermatozoa. Western blotting further confirmed the presence of a 40-kD immunoreactive protein in the membrane of mature sperm cells. Altogether , these results demonstrate that the main expression site of a subset of the large olfactory receptor gene family is not olfactory mucosa but testis. This expression correlates with the presence of the corresponding protein during sperm cell maturation, and on mature sperm cells. The pattern of expression is consistent with a role as sensor for unidentified chemicals possibly involved in the control of mammalian sperm maturation, migration, and/or fertilization

Topics: Articles
Publisher: The Rockefeller University Press
OAI identifier: oai:pubmedcentral.nih.gov:2290870
Provided by: PubMed Central
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    1. (1991). A novel multigene family may encode odorant receptors: a molecular basis for odor recognition.
    2. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    3. (1992). A rat sperm flagellar surface antigen that originates in the testis and is expressed on the flagellar surface during epididymal transit.
    4. (1993). A zonal organization of odorant receptor gene expression in the olfactory epithelium.
    5. (1988). Antibodies: a laboratory manual.
    6. (1988). Changes in lectinbinding features of ram sperm surfaces associated with epididymal maturation and ejaculation.
    7. (1992). Changes in the lectin binding sites on the testicular, epididymal, vas, and ejaculated spermatozoon surface of dog,
    8. (1986). Changes in the organization of the lipid bilayer of the plasma membrane during spermatogenesis and ¬Ępididymal maturation.
    9. (1992). Characterization of human sperm antigens and antisperm antibodies in infertile patients.
    10. (1970). Cleavage of structural proteins during assembly of the head of bacteriophage T4.
    11. (1993). Cloning and expression of odorant receptors.
    12. (1989). Cloning, sequencing and expression of the human thyrotropin (TSH) receptor: evidence for binding of autoantibodies.
    13. (1993). Coding of olfactory information: topography of odorant receptor expression in the catfish olfactory epithelium.
    14. (1992). Current protocols in immunology. Greene Publishing Associates and Wiley Interscience,
    15. (1983). Cyclic adenosine 3', 5' monophosphate, calcium and protein pbosphorylation in flagellar motility.
    16. (1979). Eiectrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    17. (1987). Electroporation for the efficient transfection of mammalian cells with DNA.
    18. (1992). Evidence for a novel splice variant of the c~ subunit of Go in rat male haploid germ cells.
    19. (1990). Evidence that human follicular fuid contains a chemoattractant for spermatozoa.
    20. (1987). Expression of mRNA into plant and animal cells. Nucleic Acids Res.
    21. (1992). Factors controlling sperm entry into the micropyles of salmonid and herring eggs.
    22. (1989). G~f: an olfactory specific-G protein involved in odorant signal transduction. Science (Wash DC).
    23. (1993). Gene knockouts of c-src, transforming growth factor El, and tenascin suggest superfluous, nonfunctional expression of proteins.
    24. (1993). Human sperm fertilizing potential in vitro is correlated with differential expression of a head-specific mannose-ligand receptor.
    25. (1990). Identification of a specialized adenylyl cyclase that may mediate odorant detection.
    26. (1991). Immunocytochemical and biochemical characterization of guanine nucleotide-biading regulatory proteins in mammalian spermatozoa.
    27. (1983). Immunogoldsilver staining: new method of immunostaining with enhanced sensitivity.
    28. (1993). Intracellular calcium increases with hyperactivation in intact, moving hamster sperm and oscillates with the flagellar beat cycle.
    29. (1981). Localization of a single sperm membrane autoantigen (RSA-1) on spermatogenic cells and spermatozoa.
    30. (1992). Members of the putative olfactory receptor gen¬Ę family are expressed in mammalian germ cells..
    31. (1989). Molecular basis of fertilization.
    32. (1988). Molecular cloning. A laboratory manual.
    33. (1993). Molecular events mediating sperm activation.
    34. (1992). Novel G protein-coupled receptors: a gene family of putative human olfactory receptor sequences.
    35. (1986). Odorant and guanine nucleotide-stimulated phosphoinositide turnover in olfactory cilia.
    36. (1985). Odorant-sensitive adenulate cyclase may reed/ate olfactory reception.
    37. (1993). Odorants differentially enhance phosphoinositide turnoverand adenylyl cyclase in olfactory receptor neuronal cultures.
    38. (1992). Precontact mammalian sperm-egg communication and role in fertilization.
    39. (1990). Primary structure and functional expression of a cyclic nucleotide-activated channel from olfactory neurons.
    40. (1990). Primary structure of cAMP-gated channel from bovine olfactory epithelium.
    41. (1988). Proacrosin/acrosin during guinea pig spermatogenesis.
    42. (1990). Protein kinase C is present in human sperm: possible role in flagellar motility.
    43. (1989). Protein pbosphorylation: the second messenger signal transducer of flagellar motility.
    44. (1990). Rapid activation of alternative second messenger pathways in olfactory cilia from rats by different odorants.
    45. (1993). receptor kinase-2 and ~-arrestin-2 as mediators of odorant-induced desensitization.
    46. (1991). Receptors for spermactivating peptides, SAP-I and SAP-fiB, on spermatozoa of sea urchins, Hemicentrotus puicherrimus and Glyptocidaris crenularis.
    47. (1988). Regional differentiation of the Vanderhacghen et al. Sperm Cell Olfactory Receptors 1451 sea urchin sperm plasma membrane.
    48. (1982). Regulation of protein phosphorylation and motility of sperm by cyclic adenosine monopbosphate and calcium.
    49. (1988). Role of epithelial cells of the rat epididymis in the disposal of the contents of cytoplasmic droplets detached from spermatozoa.
    50. (1991). Sperm attraction to a follicular factor(s) correlates with human egg fertilizability.
    51. (1993). Sperm chemotaxis during the process of fertilization in the Aseidians Ciona savignyi and Ciona intestinalis.
    52. (1989). The effects of platelet-aedvating factor on the motility of human spermatozoa.
    53. (1993). The family of genes encoding odorant receptors in the channel catfish.
    54. (1986). The odorant-sensitive adenylate cyclase of olfactory receptor cells.
    55. (1984). The potential of the immunognld-silver staining method for paraffin sections.
    56. (1980). The regulation of spermatozoa by calcium and cyclic nucleotides.
    57. (1970). The unlabeled antibody-enzyme method of immunohistochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-anti horseradish peroxidase) and its use in identification of spirochetes.

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