Location of Repository

Subcellular localization and sequence of sea urchin kinesin heavy chain: evidence for its association with membranes in the mitotic apparatus and interphase cytoplasm [published erratum appears in J Cell Biol 1991 Aug;114(4):following 863]



Kinesin was previously immunolocalized to mitotic apparatuses (MAs) of early sea urchin blastomeres (Scholey, J.M., M.E. Porter, P.M. Grissom, and J.R. McIntosh. 1985. Nature [Lond.]. 318:483-486). Here we report evidence that this MA-associated motor protein is a conventional membrane-bound kinesin, rather than a kinesin-like protein. Our evidence includes the observation that the deduced amino acid sequence of this sea urchin kinesin heavy chain is characteristic of a conventional kinesin. In addition, immunolocalizations using antibodies that distinguish kinesin from kinesin-like proteins confirm that conventional kinesin is concentrated in MAs. Finally, our immunocytochemical data further suggest that conventional kinesin is associated with membranes which accumulate in MAs and interphase asters of early sea urchin embryos, and with vesicles that are distributed in the perinuclear region of coelomocytes. Thus kinesin may function as a microtubule-based vesicle motor in some MAs, as well as in the interphase cytoplasm

Topics: Articles
Publisher: The Rockefeller University Press
OAI identifier: oai:pubmedcentral.nih.gov:2288992
Provided by: PubMed Central
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://www.pubmedcentral.nih.g... (external link)
  • Suggested articles



    1. (1989). A battery of monoclonal antibodies to kinesin heavy and light chains stain vesicle-like structures, but not microtuboles, in cultured cells.
    2. (1989). A calsequestrin-like protein in the endoplasmic reticulum of the sea urchin: localization and dynamics in the egg and first cell cycle embryo.
    3. (1984). A comprehensive set of sequence analysis programs for the VAX. Nucleic Acid Res.
    4. (1990). A kinesin-like protein required for distributive chromosome segregation in Drosophila.
    5. (1990). A monoclonal antibody against kinesin inhibits both anterograde and retrograde fast axonal transport in squid axoplasm.
    6. (1989). A three domain structure of kinesin heavy chain revealed by DNA sequence and microtubulebinding analyses.
    7. (1981). Affinity-purification of antibodies from diazotized paper blots of heterogenous protein samples.
    8. (1990). AR3, a kinesin-related gene required for yeast nuclear fusion.
    9. (1960). Aster-associated particles in the cleavage of marine invertebrate eggs.
    10. (1987). Characterization of the microtubule movement produced by sea urchin egg kinesin.
    11. (1985). Different axoplasmic proteins generate movement in opposite directions along microtubules in vitro.
    12. (1984). Differential distribution and function of microtubules and microfilaments in sea urchin coelomocytes.
    13. (1990). Differentiation of a calsequestrin-containing endoplasmic reticulum during sea urchin oogenesis.
    14. (1977). DNA sequencing with chain terminating inhibitors.
    15. (1977). Dynamic aspects of filopodial formation by reorganization of microfilaments.
    16. (1990). Evidence that the head of kinesin is sufficient for force generation and motility in vitro. Science (Wash.
    17. (1987). Gene isolation by screening lambda gtll libraries with antibodies. Methods Enzymol.
    18. (1988). Generation of single stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus.
    19. (1987). GTP-binding domain: three consensus sequence elements with distinct spacing.
    20. (1990). Identification and characterization of a gene encoding a ldnesin-like protein in Drosophila.
    21. (1985). Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility.
    22. (1989). Identification of globular mechanochemical heads of kinesin.
    23. (1985). Identification of kinesin in sea urchin eggs and evidence for its localization in mitotic spindles. Nature (Land.).
    24. (1988). Inhibition of kinesin-driven micmtubule motility by monoclonal antibodies to kinesin heavy chains.
    25. (1987). Intracellular transport using microtubule-based motors.
    26. (1988). Isolation and characThe
    27. (1979). Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    28. (1989). Isolation ofa 45kDa fragment from the ldnesin heavy chain with enhanced ATPase and microtubule-binding activities.
    29. (1988). Kinesin ATPase: rate-limiting ADP release.
    30. (1991). Kinesin heavy chain is essential for viability and neuromuscular functions in drosophila but mutants show no defects in mitosis.
    31. (1987). Kinesin is associated with a nonmierotubule component of sea urchin mitotic spindles.
    32. (1986). Kinesin: possible biological functions of a new microtubule motor. Trends Biochem.
    33. (1990). Light chains of sea urchin kinesin identified by immunoadsorption.
    34. (1990). Mediation of meiotic and early mitotic chromosome segregation in Drosophila by a protein related to kinesin.
    35. (1989). Membranes in the mitotic apparatus. In Mitosis: Molecules and Mechanisms.
    36. (1990). Microtubule and motor-dependent fusion in vitro between apical and basolateral endocytic vesicles from MDCK ceils.
    37. (1990). Microtubules, membrane traffic and cell organization.
    38. (1990). Molecular cloning of the mierotubule-associated mechanochemical enzyme dynamin reveals homology with a new family of GTP-bindlng proteins.
    39. (1989). MT-associated proteins in the sea urchin mitotic spindle.
    40. (1990). Mutation ofa gene that encodes a kinesinlike protein blocks nuclear division in A.
    41. (1988). Native structure and physical properties of bovine brain kinesin and identification of the ATP-binding subunit polypeptide.
    42. (1990). Novel potential mitotic motor protein encoded by the fission yeast cut7 + gene.
    43. (1987). Of URFs and ORFs.
    44. (1990). One motor, many tails: an expanding repertoire of force-generating enzymes.
    45. (1990). Potential roles of microtubule-associated motor molecules in cell division.
    46. (1989). Quantitative analysis of sea urchin egg kinesin-driven microtubule motility.
    47. (1990). Radial extension of macrophage tubular lysosomes supported by kinesin.
    48. (1989). Rapid amplification of eDNA ends using nested primers.
    49. (1988). Rapid production of full length ¬ĘDNAs from rare transcripts: amplification using a single genespecific oligonucleotide primer.
    50. (1962). Some structural aspects of the mitotic apparatus in sea urchin embryos.
    51. (1989). Submolecular domains of bovine brain kinesin identified by electron microscopy and monoclonal antibody decoration.
    52. (1989). The distribution, abundance and subcellular localization of kinesin.
    53. (1990). The primary structure of the squid kinesin heavy chain.
    54. (1988). The role of kinesin and other soluble factors in organeile movement along microtuboles.
    55. (1975). The role of membranes in the organization of the mitotic apparatus.
    56. (1990). Use of T7 RNA polymerase to direct expression of cloned genes.
    57. (1986). Visualization of the Ca2√∑-transport system of the mitotic apparatus of sea urchin eggs with a monoclonal antibody.
    58. (1987). What are the functions of kinesin?

    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.