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The entry of Theileria parva sporozoites into bovine lymphocytes: evidence for MHC class I involvement

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Abstract

We have examined the process of Theileria parva sporozoite entry into susceptible bovine lymphocytes and have begun to identify one of the possible molecular interactions involved in the process. The entry process involves a defined series of events and we have used a number of experimental procedures in combination with a method of quantitation to examine various aspects of this process. T. parva sporozoites are nonmotile organisms and the initial sporozoite-lymphocyte interaction is a chance event which can occur at 0-2 degrees C. All subsequent stages in the process are temperature dependent, require the participation of live intact sporozoites and host cells, and involve some cytochalasin-inhibitable rearrangement of the host cell surface membrane or cytoskeleton. Sporozoite entry can be inhibited by antibodies (mAbs) reactive with major histocompatibility complex (MHC) class I molecules (IL-A 19, IL-A 88) and with beta 2 microglobulin (B1G6), whereas mAbs reactive with MHC class II molecules (IL-A 21, J 11), and a common panleucocyte surface antigen, (IL-A 87; a bovine equivalent of CD 11a) have no effect. These results indicate that MHC class I molecules play a role in the process of T. parva sporozoite entry into bovine lymphocytes although as yet the precise role has not been determined. Once internalized within the lymphocyte, a process that takes less than 3 min at 37 degrees C, the sporozoite rapidly escapes from the encapsulating host cell membrane; a process which occurs concurrently with the discharge of the contents of the sporozoite rhoptries and microspheres. The intracytoplasmic parasite is covered by a layer of sporozoite-derived fuzzy material to which host cell microtubules rapidly become associated

Topics: Articles
Publisher: The Rockefeller University Press
OAI identifier: oai:pubmedcentral.nih.gov:2288915
Provided by: PubMed Central
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    1. (1985). A cell biologist's view of host cell recognition and invasion by malarial parasites.
    2. (1988). A component of an antigenic rhoptry complex of Plasmodium falciparum is modified after merozoite invasion.
    3. (1989). Actin filaments and the growth, movement, and spread of the intracellular parasite, Listeria monocytogenes.
    4. (1988). Activation of a Plasmodium falciparum protease correlated with merozoite maturation and erythrocyte invasion.
    5. (1988). An immunochemical analysis of class I [BoLA] molecules on the surface of bovine cells.
    6. (1978). antigens are cell surface receptors for Semliki Forest virus.
    7. (1989). Biochemical characterization of activation-associated bovine class I major histocompatibility complex antigens. Animal Genetics.
    8. (1991). Bovine accessory cell antigens.
    9. (1988). Bovine T cells, B cells, and null cells are transformed by the protozoan parasite Theileria parva.
    10. (1985). Comparative biology of intracellnlar parasitism.
    11. (1982). Correlation between effects of 24 different cytochalasins on cellular structures and cellular events and those on actin in vitro.
    12. (1988). Differentiation antigens and functional characteristics of bovine leukocytes.
    13. (1980). Does the major histocompatibility complex serve as a specific receptor for Semliki Forest virus?
    14. (1987). Effects of cytochalasin and phalloidin on actin.
    15. (1984). Electron microscope study of host-parasite interactions of Sarcocystis muris [Protozoa, Coccidia]
    16. (1977). Endocytic uptake of particles by mononuclear phagocytes and the penetration of obligate intracellular parasites.
    17. (1975). Enhanced representation of HL-A antigens on human lymphecytes after mitogenesis induced by phytohemagglutin of Epstein-Barr virus.
    18. (1984). Evidence for a common protective antigenic determinant on sporozoites of several Theileria parva strains.
    19. (1977). Evidence for differences in erythrocyte surface receptors for the malarial parasites, Plasmodium knowlesi and Plasmodium falciparum.
    20. (1984). Factors affecting the capacity of Theileria annulata sporozoites to invade bovine peripheral blood lymphecytes.
    21. (1986). Factors influencing invasion of erythrocytes by Plasmodium falciparum parasites: the effects of an N-acetyl ghcosamine neoglycoprotein and an anti-glycophorin A antibody.
    22. (1979). Fine structure of parasitic protozoa.
    23. (1983). Host cell invasion by Apicomplexa: an expression of the parasite's contractile system?
    24. (1989). Infection des lymphocytes T par TrypanoBoma cruzi. Role possible du CD3 et du HI,A-DR.
    25. (1983). Infection of mammalian cells with Theileria species.
    26. (1984). Interaction of sporozoites of Theileria parva with bovine lymphocytes in vitro. I. Early events after invasion.
    27. (1985). Invasion and early development of Sarcocystis maris [Apicomplexa, Sarcocystidae] in tissue cultures.
    28. (1986). Lamellar membranes associated with rhoptries in erythrecytic merozoites of Plasmodium knowlesi: a clue to the mechanism of invasion.
    29. (1986). Living Together: The Biology of Parasitism.
    30. (1988). Malaria parasite invasion: interactions with the red cell membrane.
    31. (1988). Mechanisms of molecular trafficking in malaria.
    32. (1986). Monoclonal antibodies identify phenotypically and functionally distinct cell types in the bovine lymphoid system.
    33. (1984). Monoclonal antibody neutralizes the sporozoite stage of different Theileria parva stocks. Parasite lmmunol.
    34. (1988). Neutral protease involved in the reinvasion of erythrocytes by Plasmodiura merozoites.
    35. (1986). Passive endocytosis of sporozoites of Theileria parva in macroplmges at 1-2"C.
    36. (1986). Plasmodium falciparum: protease inhibitors and inhibition of erythrocytic invasion.
    37. (1983). Plasmodium knowlesi: studies on invasion of Rhesus erythrocytes by merozoites in the presence of protease inlfibitors.
    38. (1985). Rhoptry secretion of membranous whorls by Plasmodium berghei sporozoites.
    39. (1991). Rhoptry secretion ofmembranous whorls by Plasmodium falciparum merozoites.
    40. (1988). Secretion of P/asrnodiumfalciparum rhoptry protein in the plasma membrane of host erythrocytes.
    41. (1989). Signal transduction events associated with exocytosis in ciliates.
    42. (1975). Structure and invasive behaviour of Plasmodium knowlesi merozoites in vitro.
    43. (1980). Synchronization of the division of Theileria macroschizonts and their mammalian host cells.
    44. (1988). The cell biology of parasite invasion and survival.
    45. (1988). The effect of protease inhibitors on Eimeria vermiformis invasion of cultured cells.
    46. (1982). The entry of sporozoites of Theileria parva into bovine lymphecytes in vitro. Electron microscopic observations.
    47. (1985). The entry of sporozoites of Theileria parva into bovine lymphocytes in vitro. Immunoelectron microscopic observations.
    48. (1989). The fine structure of secretion by Plasmodium knowlesi merozoites during red blood cell invasion.
    49. (1986). The major surface protein of Leishmania promastigotes is a protease.
    50. (1977). The preservation of ultrastructure in saturated phosphatidyl choline by tannic acid in model systems and type II pneumocytes.
    51. (1989). The promastigote surface protease of Leishmania: pH optimum and effects of protease inhibitors.
    52. (1987). The promastigote surface protease ofLeishmania.
    53. (1988). The role of lipids in Plasmodium falciparura invasion of erythrocytes: a coordinated biochemical and microscopic analysis.
    54. (1989). Theileria parva in cattle: characterization of infected lymphocytes and the immune responses they provoke.
    55. (1989). Theileriasis: a comprehensive review.
    56. (1987). Theileriidae. In
    57. (1990). Transfection into mouse L cells of genes encoding two serologically and functionally distinct bovine class I MHC molecules from a MHC-homozygous animal: evidence for a second class I locus in cattle.

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