Location of Repository

The glycosyl phosphatidylinositol anchor is critical for Ly-6A/E- mediated T cell activation

By 

Abstract

Ly-6E, a glycosyl phosphatidylinositol (GPI)-anchored murine alloantigen that can activate T cells upon antibody cross-linking, has been converted into an integral membrane protein by gene fusion. This fusion product, designated Ly-6EDb, was characterized in transiently transfected COS cells and demonstrated to be an integral cell surface membrane protein. Furthermore, the fusion antigen can be expressed on the surface of the BW5147 class "E" mutant cell line, which only expresses integral membrane proteins but not GPI-anchored proteins. The capability of this fusion antigen to activate T cells was examined by gene transfer studies in D10G4.1, a type 2 T cell helper clones. When transfected into D10 cells, the GPI-anchored Ly-6E antigen, as well as the endogenous GPI-anchored Ly-6A antigen, can initiate T cell activation upon antibody cross-linking. In contrast, the transmembrane anchored Ly-6EDb antigen was unable to mediate T cell activation. Our results demonstrate that the GPI-anchor is critical to Ly-6A/E-mediated T cell activation

Topics: Articles
Publisher: The Rockefeller University Press
OAI identifier: oai:pubmedcentral.nih.gov:2288838
Provided by: PubMed Central
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://www.pubmedcentral.nih.g... (external link)
  • Suggested articles

    Preview

    Citations

    1. A glycophospholipid anchor is required for Qa-2-mediated T cell activation.
    2. (1988). A role in transmembrane signaling for the cytoplasmic domain of the CD2 T lymphocyte surface antigen.
    3. (1986). Anchoring of membrane proteins via phosphatidylinositol is deficient in two classes of Thy-1 negative mutant lymphoma cells.
    4. (1986). Biochemical characterization of the murine activated lymphocyte alloantigen Ly6E. 1 controlled by the Ly6 locus.
    5. (1987). Biochemistry of the glycosyl-phosphatidylinosito] membrane protein anchors.
    6. (1989). Biosynthesis of a phosphatidylinositolglycan-linked membrane protein: signals for posttranslational processing of the Ly-6E antigen.
    7. (1988). Cell-surface anchoring of proteins via glycosyl-phosphatidylinositol structure.
    8. (1984). Conversion of a secretory protein into a transmembrane protein results in its transport to the Golgi complex but not to the cell surface.
    9. (1985). Glycosylation allows cell-surface transport of an anchored secretory protein.
    10. (1986). Hydrophilic anchor-deficient Thy-1 is secreted by a class E mutant T lymphoma.
    11. (1986). Induction ofT cell activation by monoclonal anti-Thy-I antibodies.
    12. (1986). Isolation of a murine Ly-6 eDNA reveals a new multigene family.
    13. (1989). Kinetic analysis of Ly-6 gene induction in a T lymphoma by interferons and interleukin 1, and demonstration of Ly-6 inducibility in diverse cell types.
    14. (1990). Membrane assembly in vitro: synthesis, glycosylation, and asymmetric insertion of a tmnsmembrane protein.
    15. (1989). Mouse hematopoietic stem-cell antigen is a membrane of the Ly-6 antigen family.
    16. (1985). Rapid and efficient site-specific mutagenesis without phenotypic selection.
    17. (1987). Removal of lymphocyte surface molecules with phosphatidylinositol-specific phospholipase C: effects on mitogen responses and evidence that ThB and certain Qa antigens are membrane-anchored via phosphatidylinositol.
    18. (1988). SR~ promoter: an efficient and versatile mammalian cDNA expression system composed of the simian virus 40 early promoter and the R-U5 segment of human T-cell leukemia virus type 1 long terminal repeat.
    19. (1987). Stimulation ofT cells via the TAP molecule, a member in a family of activating proteins encoded in the Ly-6 locus.
    20. (1988). Structural and functional roles of glycosyl-phosphatidylinositol in membranes.
    21. (1986). Structural characterization of the TAP molecule: a phosphatidylinositol-linked glycoprotein distinct from the T cell receptor/T3 complex and Thy-1.
    22. (1988). T cell receptor/CD3 negative variants are unresponsive to stimulation through the Ly-6 encoded molecule,
    23. (1988). TAP transcription and phosphatidylinositol linkage mutants are defective in activation through the T cell receptor.
    24. (1989). The Co-receptor function of murine CD4.
    25. (1989). The Ly-6 locus: a multigene family encoding phosphatidylinositol anchored membrane proteins concerned with T-cell activation,
    26. (1988). Thy- 1- and Ly6-mediated lymphokine production and growth inhibition of a T cell hybridoma require co-expression of the T cell antigen receptor complex.
    27. (1986). Two types of routine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins.

    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.