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u.ac.jp Anuran Calcitonins are Diverse in Lower Vertebrates

By Hidenori Sakamoto, Yuichi Sasayama and Kunihiko Ueda

Abstract

ABSTRACT—We investigated the nucleotide sequences of cDNA fragments coding calcitonin from ultimobranchial glands in 2 species of urodelans (1 salamander and 1 newt) and 4 species of anurans (1 toad and 3 frogs) by the reverse transcription polymerase chain reaction (RT-PCR) method and rapid amplifica-tion of cDNA ends (RACE)-PCR method. The salamander and newt calcitonins were each 97 % and 94% similar to the lungfish and caiman calcitonins that we have already reported, in the amino acid sequences. However, anuran calcitonins were not only dissimilar (63–81%) to the lungfish and caiman calcitonins but also diversified (59–91%) even among anurans. The sequence identity of toad calcitonin was always low (59–66%) among anurans

Year: 2016
OAI identifier: oai:CiteSeerX.psu:10.1.1.1000.9518
Provided by: CiteSeerX
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