Location of Repository

Analysis of Gene Expression in Normal and Cancer Cells Exposed to γ-Radiation

By M. Ahmad Chaudhry

Abstract

The expression of many genes is modulated after exposure to ionizing radiation. Identification of specific genes may allow the determination of pathways important in radiation responses. We previously identified modulation of the expression of several genes in response to ionizing radiation treatment. In the present study, we monitored the expression of RGS1, CC3, THBS1, vWF, MADH7, and a novel gene encoding a secreted protein in irradiated Jurkat, TK6, HeLa, and HFL1 cells. The RGS1 is involved in G-protein signaling pathway, CC3 belongs to the complement system, THBS1 is a component of the extracellular matrix, vWF takes part in blood coagulation, and MADH7 is a member of the TGF-β signal transduction pathway. Our objective was to find similarities and differences in the expression of these genes in ionizing radiation-exposed diverse cell types. RGS1 was downregulated in Jurkat cells but was upregulated in TK6 and HFL1 cells. The expression of CC3 was repressed in Jurkat and HFL1 cells but was induced in TK6 and HeLa cells. THBS1 was downregulated in irradiated TK6 and HFL1 cells. vWF was induced in radiation-exposed HeLa cells, but its expression was downregulated in Jurkat cells. The expression of MADH7 was induced in all the cell types examined. These results indicate cell specific modulation of gene expression and suggest the involvement of different pathways in cellular response to radiation treatment in different cells

Topics: Research Article
Publisher: Hindawi Publishing Corporation
OAI identifier: oai:pubmedcentral.nih.gov:2276817
Provided by: PubMed Central
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://www.pubmedcentral.nih.g... (external link)
  • Suggested articles

    Preview

    Citations

    1. (2006). a j u m d e r ,D . - I .J e o u n g ,e ta l . ,“ O r g a n - s p e c i fi c gene expressions in C57BL/6 mice after exposure to low-dose radiation,”
    2. (2006). Base excision repairbyhNTH1andhOGG1:atwoedgedswordintheprocessingofDNA damage inγ-irradiatedhumancells,”DNARepair,
    3. (2006). Bystander effect: biological endpoints and microarray analysis,”
    4. Characterization of ATF3 induction after ionizing radiation in human skin cells,” JournalofRadiationResearch,vol.46,no.4,pp.379–385,2005.
    5. (2003). Differential responses of stress genes to low dose-rate γ irradiation,”
    6. (2004). Diminished cellular and humoral immunity in workers occupationally exposed to low levels of ionizing radiation,”
    7. Dissimilar genome response to acute and chronic low-dose radia t i o ni nm a l ea n df e m a l em i c e ,
    8. (2004). Evolution of the complement system,”
    9. (2006). Expression and activation of
    10. (2005). Extremely low dose ionizing radiation up-regulates CXC chemokines in normal human fibroblasts,”
    11. (2003). Fornace Jr., “Functional genomics as a window on radiation stress signaling,”
    12. (2005). Identification of a novel ionizing radiation-induced nuclease, AEN, and its functional characterization in apoptosis,”
    13. (2006). In vivo nitric oxide production and iNOS expression in X-ray irradiated mouse skin,”
    14. (2005). Integrative radiation carcinogenesis: interactions between cell and tissue responses to DNA damage,”
    15. (1996). Ionizing irradiation increases transcription of the von Willebrand factor gene in endothelial cells,”
    16. (2006). Ionizing radiation enhances the expression of the nonsteroidal antiinflammatory drug-activated gene (NAG1) by increasing the expression of TP53 in human colon cancer cells,”
    17. (2004). l o k o v ,T .C r i s w e l l ,K .S .L e s k o v ,S .A r a k i ,L .M a y o ,a n dD
    18. (2004). Metabolic oxidation/reduction reactions and cellular responses to ionizing radiation: a unifying concept in stress response biology,” Cancer and Metastasis Reviews,
    19. (2005). Microarray analysis of the transcriptional response to single or multiple doses of ionizing radiation in humansubcutaneousfibroblasts,”RadiotherapyandOncology,
    20. (2004). Microarray analysis of UVB-regulated genes in keratinocytes: downregulation of angiogenesis inhibitor thrombospondin-1,”
    21. (2004). New insights into TGF-β-Smad signalling,”
    22. (1995). o n g ,C . - S .C h i a n g ,I .L .C a m p b e l l ,J . - R .S u n ,H .R . W i t h e r s ,a n dW .H .M c B r i d e ,“ I n d u c t i o no fa c u t ep h a s eg e n e expression by brain irradiation,”
    23. (2006). Radiation-induced gene expression profile of human cells deficient in 8-hydroxy-2 -deoxyguanine glycosylase,”
    24. (2001). Response of base excision repair enzymes to complex
    25. (2002). Stress-gene induction by low-dose γ irradiation,”
    26. (2006). Sz¨ u t s ,a n dT .K r u d e ,“ I n d u c t i o no fC
    27. (2006). Temporal cDNA microarray analysis of gene expression in human hepatocellular carcinoma upon radiation exposure,”
    28. (2004). The role of thrombospondin-1 in human disease,”
    29. (2006). u g i m o t o ,A .G r o m l e y ,a n dC .J .S h e r r ,“ Hzf, a p53-responsive gene, regulates maintenance of the G2 phase checkpoint induced by DNA damage,”

    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.