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Rapid Diagnosis of Human Brucellosis by Quantitative Real-Time PCR: a Case Report of Brucellar Spondylitis▿

By Amalia Navarro-Martínez, Elena Navarro, Maria Jesús Castaño and Javier Solera


Blood samples and a bone biopsy specimen from one patient diagnosed with spondylodiscitis of unknown etiology were analyzed by quantitative real-time PCR to detect Brucella melitensis. The high sensitivity and specificity of this assay allowed the diagnosis of brucellar spondylitis within 24 h, a result that we were unable to obtain through the use of conventional methods

Topics: Case Reports
Publisher: American Society for Microbiology (ASM)
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Provided by: PubMed Central
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