Article thumbnail

CHEMICAL CHARACTERISTICS OF COLLAGEN EXTRACT FROM SCAPULA OF BALI CATTLE (Os Scapula) PRODUCED USING DIFFERENT EXTRACTANT

By Muhammad Irfan Said, Effendi Abustam, Abd.Wahid Wahab, Sartini and Amriana Hifizah

Abstract

The structure of cattle bone is rich of protein collagen compounds that are strongly bound to the minerals calcium (Ockerman and Hansen, 2000). Collagen is a structural protein that is only produced by the body tissues of cattle (Zeugolis et al., 2008) and recently, it has been widely used as a food supplement to prevent bone disease (osteoporosis), joint pain (osteoarthritis) and premature aging (Rogart et al., 1999 ; Tian et al., 2001). Until now the use of abattoir waste such as bone as the cheap collagen protein source in fact has not been widely explored. \ud The exploration of bone waste as a source of collagen is possible, because the collagen compound contained in cow bones appeared to have similarities in terms of chemical composition, morphology, distribution, function and pathology with the collagen compound found in the human body (Junqueira et al., 1998).The exploration process of bioactive compounds such as collagen extract derived from bovine bone until now has not been much publicized yet. Therefore, this exploratory research has been conducted in the form of the optimization of the production processes of bone collagen compounds, especially Bali cattle bones in the shoulder blade (os scapula) in the form of extracts instant readily consume. The results of the initial study showed that the shoulder blade (os scapula) of Bali cattle have the potential for higher protein content (39.4%) compared to other parts such as the spinal bones (os vertebrae) (37.56%), thigh bone (os femur) (24.06%) and lower leg bone (os tibia-fibula) (Said et al., 2012).\ud The optimization of the production process allows the bioactive compounds in the form of collagen can be done through an appropriate extractant engineering use. The use of an appropriate extractant in the production process of collagen extracts, will possibly produce the maximum quantity and quality of the product (Wang et al., 2008 ; Zeugolis et al., 2008). This study aimed to evaluate the chemical properties of the product of extract collagen from the raw materials of shoulder blade (os scapula) of Bali cattle through the application of some type of extractants in the production process.Collagen is a hydrocolloid products of animal protein resulted in partial hydrolysis. Cow bones abattoir by-product is a product that is rich in collagen proteins, thereby it???s potentially as a source of raw materials. The protein collagen is now widely used as a dietary supplement. This study aimed to evaluate the chemical properties of the extract collagen product from os scapula of Bali cattle through the application of some types of extractant in the production process. Laboratory experimental method was used in this study. A total of four types of extractant was applied, namely: (1) distilled water, (2) ethanol 60% (v/v), (3) CH3COOH 0.5 M and (4) Ca(OH)2 0.5 M. The pattern of Complete Randomized Design (RAL) of Undirectional pattern and the analysis of variance was used as the study design and data analysis, while Duncan's Multiple Range Test (DMRT) was used as a further test if it was significant. The results showed that the use of different extractant extracts on the collagen production processes using raw materials from os scapula of Bali cattle affected (p<0.05) the value of the yield, moisture content, protein content, and calcium levels, but there was no significant effect (p>0.05) on lipid levels and phosphorus levels. Extract collagen from os scapula of Bali cattle produced using four types of extractants have functional properties identical functional groups based on the presence of CO, C-O, OH, CH, and N-H bond

Topics: collagen extract ; os scapula ; Bali cattle, extractant, chemical aspect
Publisher: Asian Network for Scientifict Information (ANSI)
Year: 2015
DOI identifier: 10.3923/pjn.2015.174.179
OAI identifier: oai:repository.unhas.ac.id:123456789/13472
Journal:

Suggested articles


To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.