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Role of carbon dioxide and ion transport in the formation of sub-embryonic fluid by the blastoderm of the Japanese quail

By G.V. Latter and Glenn K. Baggott

Abstract

1. The explanted blastoderm of the Japanese quail was used to explore the role of ions and carbon dioxide in determining the rate of sub-embryonic fluid (SEF) production between 54 and 72 h of incubation.\ud 2. Amiloride, an inhibitor of Na+/H+ exchange, at concentrations of 10-3 to 10-6 M substantially decreased the rate of SEF production when added to the albumen culture medium. N-ethylmaleimide, an inhibitor of V type H+ ATPase, also decreased this rate but only to a small extent at the highest dose applied, 10-3 M. Both inhibitors had no effect on SEF production when added to the SEF. 3. The inhibitors of cellular bicarbonate and chloride exchange, 4-acetamido-4-'isothiocyano-2, 2-'disulphonic acid (SITS) and 4,4'diisothiocyanostilbene-2,2-'disulphonic acid (DIDS), had no effect upon SEF production.\ud 4. Ouabain, an inhibitor of Na+/K+ ATPase, decreased SEF production substantially at all concentrations added to the SEF (10-3 to 10-6 M). Three sulphonamide inhibitors of carbonic anhydrase, acetazolamide, ethoxzolamide and benzolamide, decreased SEF production when added to the SEF at concentrations of 10-3 to 10-6 M. Benzolamide was by far the most potent. Neither ouabain nor the sulphonamides altered SEF production when added to the albumen culture medium.\ud 5. Using a cobalt precipitation method, carbonic anhydrase activity was localised to the endodermal cells of the area vasculosa. The carbonic anhydrase activity was primarily associated with the lateral plasma membranes, which together with the potent inhibitory effect of benzolamide, suggests the carbonic anhydrase of these cells is the membrane-associated form, CA IV.\ud 6. The changes in SEF composition produced by inhibitors were consistent with the production of SEF by local osmotic gradients.\ud 7. It is concluded that a Na+/K+ ATPase is located on the basolateral membranes of the endodermal cells of the area vasculosa , and that a sodium ion/hydrogen ion exchanger is located on their apical surfaces. Protons for this exchanger would be provided by the hydration of CO2 catalysed by the membrane-associated carbonic anhydrase. Furthermore, it is proposed that the prime function of the endodermal cells of the area vasculosa is the production of SEF

Topics: bcs
Publisher: Science Reviews 2000
Year: 2002
OAI identifier: oai:eprints.bbk.ac.uk.oai2:224

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Citations

  1. (1955). A new technique for the cultivation of the chick embryo in vitro.
  2. (1982). Amiloride: a molecular probe of sodium transport in tissues and cells.
  3. (1982). Amiloride: a molecular probe of sodium transport in tissues and cells. A merican
  4. (1999). Animal plasma membrane energization by proton-motive V-ATPases. doi
  5. (1987). Basolateral membrane Cl -/HCO 3- exchange in the rat proximal convoluted tubule: Na-dependent and independent modes. doi
  6. (1987). Basolateral membrane Cl -/HCO3 - exchange in the rat proximal convoluted tubule: Na-dependent and independent modes. doi
  7. (1982). Benzolamide. A renal carbonic anhydrase inhibitor, doi
  8. (1967). Biochemistry of the Avian Embryo doi
  9. (1968). Carbon dioxide during storage of chicken and turkey eggs. doi
  10. (1991). Carbonic anhydrase and proton secretion in turtle bladder mitochondrial-rich cells.
  11. (1991). Carbonic anhydrase and proton secretion in turtle bladder mitochondrial-rich cells. A merican
  12. (1986). Carbonic anhydrase inhibition and cell volume regulation in Necturus gallbladder. doi
  13. (1967). Carbonic anhydrase: chemistry, physiology and inhibition . doi
  14. (1967). Carbonic anhydrase: chemistry, physiology and inhibition. doi
  15. (1991). Carbonic anhydrases in the kidney, in: doi
  16. (1990). Diuretic compounds structurally related to furosemide. doi
  17. (1992). Effect of turning upon the sub-embryonic fluid and albumen of the egg of the Japanese quail. doi
  18. (1995). Effects of temperature and carbon dioxide on albumen characteristics, weight loss, and early embryonic mortality of long stored hatching eggs. doi
  19. (1997). Egg handling and storage. doi
  20. (1991). Egg water movements during incubation, in:
  21. (1991). Epithelial Na + channels. doi
  22. (1994). High- activity carbonic anhydrase isoenzyme doi
  23. (1994). High-activity carbonic anhydrase isoenzyme (CA 11) in human gallbladder epithelium. doi
  24. (1968). Histochemical demonstrat ion of carbonic anhydrase activity in some epithelia noted for active transport. doi
  25. (1968). Histochemical demonstration of carbonic anhydrase activity in some epithelia noted for active transport. doi
  26. (1992). Histochemical localization of carbonic anhydrase in the testis and epididymis of the rabbit. doi
  27. (1995). Human carbonic anhydrases and carbonic anhydrase deficiencies. doi
  28. (1984). Immunohistochemical localisation of human carbonic anhydrase isoenzymes. doi
  29. (1989). Importance of sub-em bryonic fluid and albumen in the embryo’s response to turning of the egg during incubation. doi
  30. (1989). Importance of sub-embryonic fluid and albumen in the embryo’s response to turning of the egg during incubation. doi
  31. (1976). Intracellular localisation of carbonic anhydrase in the frog nephron. doi
  32. (1989). Ion transport across the gallbladder epithelium. doi
  33. (1980). Kinetics, equilibrium and inhibition in the Hansson histochemical procedure for carbonic anhydrase: a validation of the method. doi
  34. (1996). Localisation of carbonic anhydrase in the sperm storing regions of the domestic hen oviduct. doi
  35. (1990). Localization of membrane-associated carbonicanhydrase type- I V in kidney epithelial-cells. doi
  36. (1990). Localization of membrane-associated carbonicanhydrase type-IV in kidney epithelial-cells. doi
  37. (1990). Mechanisms of chloride transport in secretory epithelia. doi
  38. (1996). Membrane-bound carbonic anhydrase IV is expressed in the luminal plasma membrane of the human gallbladder. doi
  39. (1957). Ontogenetic changes in the freezing point and sodium and potassium content of the subgerminal fluid and blood plasma of the chick embryo. doi
  40. (1979). Osmotic water flow in leaky epithelia. doi
  41. (1958). Relationship between temperature and carbon dioxide loss from shell eggs. doi
  42. (1989). Renal Membrane-Bound Carbonic-Anhydrase -Purification and Properties. doi
  43. (1982). Selectivity properties of channels induced by a reconstituted membrane-bound carbonic anhydrase. doi
  44. (1958). Shell porosity of chicken eggs. doi
  45. (1958). Shell porosity of chicken eggs. 1. CO2 loss and CO2 content of infertile eggs during storage. doi
  46. (1983). Sodium transport and the control of epiblast polarity in the early chick embryo.
  47. (1969). st edition doi
  48. (1993). Structure of the endodermal epithelium of the chick yolk sac during early stages of development. doi
  49. (1991). The acid-base status of the sub-embryonic fluid of the Japanese quail, Coturnix coturnix japonica , in:
  50. (1991). The acid-base status of the sub-embryonic fluid of the Japanese quail, Coturnix coturnix japonica, in:
  51. (1960). The Avian Embryo doi
  52. (1991). The carbonic anhydrases. Overview of their importance in cellular physiology and in molecular genetics, doi
  53. (1924). The cultivat ion of tissues in saline embryonic juices. doi
  54. (1956). The formation of sub-blastodermic fluid in hens' eggs.
  55. (1956). The gaseous environment of the chick embryo in relation to its development and hatchability. 1. Effect of carbon dioxide and oxygen levels during the first four days of incubation upon hatchability. doi
  56. (1965). The gaseous environment of the chick embryo in relation to its development and hatchability. 2. Effect of carbon dioxide and oxygen levels during the period of the fifth through the eighth days of incubation. doi
  57. (1997). The key role of the H + V-ATPase in acid-base balance and Na + transport processes in frog skin.
  58. (1988). The kinetics of HCO 3− synthesis related to fluid secretion, pH control, doi
  59. (1988). The kinetics of HCO3 − synthesis related to fluid secretion, pH control, and CO2 elimination. doi
  60. (1989). The renal proximal tubule: A model for diversity of anion exchangers and stilbene-sensitive anion transporters. doi
  61. (1991). The sub-embryonic fluid of the egg of the domestic fowl and its relationship to the early development of the embryo in:
  62. (1981). Transport across endodermal cells of the chick yolksac during early stages of development. doi
  63. (1980). Water and ionic fluxes inside the egg. doi

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