Location of Repository

Induced hyperlipaemia and immune challenge in locusts

By Lisa M. Mullen, M.E. Lightfoot and Graham J. Goldsworthy


Injections of immunogens, such as β-1,3-glucan or lipopolysaccharide (LPS), bring about a marked hyperlipaemia with associated changes in lipophorins and apolipophorin-III in the haemolymph of Locusta migratoria. These changes are similar to those observed after injection of adipokinetic hormone (AKH). The possibility that endogenous AKH is released as part of the response to these immunogens is investigated using passive immunisation against AKH-I, and measurement of AKH-I titre in the haemolymph after injection of immunogens. The data presented show that, despite the similarity of the changes brought about by the presence of immunogens in the haemolymph to those brought about by AKH, there is no release of endogenous AKH after injection of laminarin or LPS. A direct effect of the immunogens on release of neutral lipids by the fat body cannot be demonstrated in vitro, and the mechanism by which hyperlipaemia is induced during immune challenge remains uncertain

Topics: bcs
Publisher: Elsevier
Year: 2004
OAI identifier: oai:eprints.bbk.ac.uk.oai2:239

Suggested articles



  1. 1 990. Adipoki n e t i c Hormone Control s Lipid Metabo l i s m in Adults and Carbohyd r a t e Metaboli s m in Larvae of Manduca Sexta .
  2. (2000). A beta 1,3-gluc an recognition protei n from an insect, Manduca sexta, agglut i n a t e s microo r g a n i s ms and activat e s the phenolo x i d a s e cascade . Journal of Biologi c a l Chemist r y 275,
  3. (1993). A Novel Role of Periplaneta Lectin as an Opsonin to Recognize 2-Keto-3-Deoxy Octona t e Residu e s of Bacter i a l Lipopo l y s a c c h a r i d e s . Compar a t i v e Bioche mi s t r y and Physio l o g y B-Bioc h e mi s t r y & Molecu l a r Biolog y 106,
  4. (2002). A quantitative study of adipoki n e t i c hormon e of the firebug , Pyrrhocoris apterus . Journa l of Insect Physiol o g y 48, doi
  5. (1990). A s h i d a doi
  6. (1973). A si mpli f i e d method for the quanti t a t i v e assay of small amount s of protei n in biolog i c a l materia l s . Analyti c a l Biochemi s t r y 51,
  7. (2002). Adipoki n e t i c hormone enhanc e s lami na r i n and bacter i a l lip op o l y s a c c ha r i d e - i n d u c e d activa t i o n of the prophe n o l o x i d a s e cascad e in the Africa n migrat o r y locust , Locusta migratoria.
  8. (1994). Adipoki n e t i c Hormone s Inhibit RNA-Synt h e s i s in the Fat-Bod y of Adult Locusta-Migratoria. Insect Neuroc h e mi s t r y and Neurophy s i o l o g y
  9. (2002). Adipokinetic hormones con ce n t r a t i o n s in the haemol y mp h of Schistocerca gregaria, measur e d by radioi m mu n o a s s a y . Insect Biochemi s t r y and Molecul a r
  10. (1988). Antibodies: A Laboratory Manual. doi
  11. (2002). Apolipophorin-III affects the activit y of the haemocy t e s of Galleria mellonella larvae. doi
  12. (2001). B.,Wie s n e r , A.,
  13. (2001). C.,Wie s n e r , A., doi
  14. (1985). C.M.,Wh e e l e r , C.H.,
  15. (2003). Chandra k a n t , S., Opoku-Wa r e , K.,
  16. (2003). Change s in lipopho r i n s are related to the activa t i o n of phenol o x i d a s e in the haemol y mp h of Locusta migratoria in response to injection of immunogens . Insect Bioche mi s t r y and Molecu l a r Biolog y 33,
  17. (1999). Differ e n t i a l locati o n of peptide hormon e s in the secretory pathway of insect adipoki n e t i c cells.
  18. (1995). Goldsw o r t h y
  19. (1979). Goldswo r t h y , G.J.,
  20. (1974). Goldswor t h y , G.J.,
  21. (1999). H.B.,Kan o s t , M.R., doi
  22. (1997). Haemol y mp h protei n s of larvae of Galleria mellonella detoxify endotoxins of the insect pathoge n i c bact e ri a Xenorhabdus nematophilus (Entero ba c t e r i a c e a e ) . doi
  23. (1990). Hor monal control of f light metabol i s m in locusts .
  24. (1997). Isolated apolipophorin III from Galleria mellonella stimula t e s the immune reacti o n s of this insect. doi
  25. (1989). L.,Boema r e , N., doi
  26. (1997). M.R.,T r e n c z e k , T., doi
  27. (1999). On the release of the three locust ( Locusta migratoria) adipok i n e t i c hormon e s : Effect of crustac e a n cardi o a c t i v e pepti d e and inhibi t i o n by sugars . Zeitsc h r i f t Fur Naturf o r s c h u n g C-a
  28. (2003). Opoku-Wa r e , K., Chandrak a n t , S.,
  29. (1993). P.,Va n d e r h o r s t
  30. Physiolo g y 14195 Berlin, Ge rmany Free Univ Berlin,
  31. (2000). Plasma t o c y t e spread i n g peptid e (PSP1) and growth blocking peptide (GBP ) are multi f u n c t i on a l homol o g s . Journ a l of Insect Physiolo g y 46,
  32. (1990). Purification and characterization of lipopol y s a c c h a r i d e - b i n d i n g protei n from he molym p h of Ame r ica n cockro a c h Periplaneta americana . Europea n Journal of Biochemi s t r y 190,
  33. (2004). Ra tcli f f e
  34. (1998). Recent advances in research on the insect prophe n o l o x i d a s e cascad e .
  35. (1998). Role of the prophen o l o x i d a s e - a c t i v a t i n g system in inverte b r a t e immunit y . Current Opinion in Immu nology
  36. (1991). Slotboo m, A.J.,Be e n a k k e r s
  37. (1997). Structur e s , assay s and recep t or s for locus t adipoki n e t i c hormo n e s . Comp a r a t i v e Bioch e mi s t r y and Physio l o g y B-Bioc h e mi s t r y & Molecul a r Biology 117,

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.